Your browser doesn't support javascript.
loading
Rapid, Multiplexed, and Enzyme-Free Nucleic Acid Detection Using Programmable Aptamer-Based RNA Switches.
Yan, Zhaoqing; Eshed, Amit; Tang, Anli A; Arevalos, Nery R; Ticktin, Zachary M; Chaudhary, Soma; Ma, Duo; McCutcheon, Griffin; Li, Yudan; Wu, Kaiyue; Saha, Sanchari; Alcantar-Fernandez, Jonathan; Moreno-Camacho, Jose L; Campos-Romero, Abraham; Collins, James J; Yin, Peng; Green, Alexander A.
Afiliación
  • Yan Z; Department of Biomedical Engineering, Boston University, Boston, MA, USA.
  • Eshed A; Molecular Biology, Cell Biology & Biochemistry Program, Graduate School of Arts and Sciences, Boston University, Boston, MA, USA.
  • Tang AA; Biological Design Center, Boston University, Boston, MA 02215, USA.
  • Arevalos NR; Department of Biomedical Engineering, Boston University, Boston, MA, USA.
  • Ticktin ZM; Biological Design Center, Boston University, Boston, MA 02215, USA.
  • Chaudhary S; Biodesign Center for Molecular Design and Biomimetics at the Biodesign Institute, Arizona State University, Tempe, AZ, USA.
  • Ma D; School of Molecular Sciences, Arizona State University, Tempe, AZ, USA.
  • McCutcheon G; Department of Biomedical Engineering, Boston University, Boston, MA, USA.
  • Li Y; Biological Design Center, Boston University, Boston, MA 02215, USA.
  • Wu K; Biodesign Center for Molecular Design and Biomimetics at the Biodesign Institute, Arizona State University, Tempe, AZ, USA.
  • Saha S; Biodesign Center for Molecular Design and Biomimetics at the Biodesign Institute, Arizona State University, Tempe, AZ, USA.
  • Alcantar-Fernandez J; School of Molecular Sciences, Arizona State University, Tempe, AZ, USA.
  • Moreno-Camacho JL; Biodesign Center for Molecular Design and Biomimetics at the Biodesign Institute, Arizona State University, Tempe, AZ, USA.
  • Campos-Romero A; School of Molecular Sciences, Arizona State University, Tempe, AZ, USA.
  • Collins JJ; Department of Biomedical Engineering, Boston University, Boston, MA, USA.
  • Yin P; Biological Design Center, Boston University, Boston, MA 02215, USA.
  • Green AA; Biodesign Center for Molecular Design and Biomimetics at the Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Chem ; 10(7): 2220-2244, 2024 Jul 11.
Article en En | MEDLINE | ID: mdl-39036067
ABSTRACT
Rapid, simple, and low-cost diagnostic technologies are crucial tools for combatting infectious disease. We describe a class of aptamer-based RNA switches or aptaswitches that recognize target nucleic acid molecules and initiate folding of a reporter aptamer. Aptaswitches can detect virtually any sequence and provide an intense fluorescent readout without intervening enzymes, generating signals in as little as 5 minutes and enabling detection by eye with minimal equipment. Aptaswitches can be used to regulate folding of seven fluorogenic aptamers, providing a general means of controlling aptamers and an array of multiplexable reporter colors. Coupling isothermal amplification reactions with aptaswitches, we reach sensitivities down to 1 RNA copy/µL in one-pot reactions. Application of multiplexed all-in-one reactions against RNA from clinical saliva samples yields an overall accuracy of 96.67% for detection of SARS-CoV-2 in 30 minutes. Aptaswitches are thus versatile tools for nucleic acid detection that are readily integrated into rapid diagnostic assays.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chem Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Chem Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos