Your browser doesn't support javascript.
loading
Glutamatergic signaling from melanin-concentrating hormone-producing neurons: A requirement for memory regulation, but not for metabolism control.
Pham, Xuan Thang; Abe, Yoshifumi; Mukai, Yasutaka; Ono, Daisuke; Tanaka, Kenji F; Ohmura, Yu; Wake, Hiroaki; Yamanaka, Akihiro.
Afiliación
  • Pham XT; Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya 464-8601, Japan.
  • Abe Y; Department of Neural Regulation, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
  • Mukai Y; Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
  • Ono D; Department of Psychiatry, Hanoi Medical University, Hanoi 100000, Vietnam.
  • Tanaka KF; Division of Brain Sciences, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo 160-8582, Japan.
  • Ohmura Y; Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya 464-8601, Japan.
  • Wake H; Department of Neural Regulation, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
  • Yamanaka A; Japan Society for the Promotion of Science, Tokyo 102-0083, Japan.
PNAS Nexus ; 3(7): pgae275, 2024 Jul.
Article en En | MEDLINE | ID: mdl-39035036
ABSTRACT
Melanin-concentrating hormone-producing neurons (MCH neurons), found mainly in the lateral hypothalamus and surrounding areas, play essential roles in various brain functions, including sleep and wakefulness, reward, metabolism, learning, and memory. These neurons coexpress several neurotransmitters and act as glutamatergic neurons. The contribution of glutamate from MCH neurons to memory- and metabolism-related functions has not been fully investigated. In a mouse model, we conditionally knocked out Slc17a6 gene, which encodes for vesicular glutamate transporter 2 (vGlut2), in the MCH neurons exclusively by using two different

methods:

the Cre recombinase/loxP system and in vivo genome editing using CRISPR/Cas9. Then, we evaluated several aspects of memory and measured metabolic rates using indirect calorimetry. We found that mice with MCH neuron-exclusive vGlut2 ablation had higher discrimination ratios between novel and familiar stimuli for novel object recognition, object location, and three-chamber tests. In contrast, there was no significant change in body weight, food intake, oxygen consumption, respiratory quotient, or locomotor activity. These findings suggest that glutamatergic signaling from MCH neurons is required to regulate memory, but its role in regulating metabolic rate is negligible.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: PNAS Nexus Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: PNAS Nexus Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido