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Effects of Ripasudil Hydrochloride on Epithelial Repair in a Mouse Cornea.
Takada, Yukihisa; Yasuda, Shingo; Sumioka, Takayoshi; Okada, Yuka; Tamura, Tadahiko; Yamanaka, Osamu; Saika, Shizuya.
Afiliación
  • Takada Y; Department of Ophthalmology, Wakayama Medical University School of Medicine, Wakayama, Japan.
  • Yasuda S; Department of Ophthalmology, Wakayama Medical University School of Medicine, Wakayama, Japan.
  • Sumioka T; Department of Ophthalmology, Wakayama Medical University School of Medicine, Wakayama, Japan.
  • Okada Y; Department of Ophthalmology, Kihoku Hospital, Wakayama Medical University School of Medicine, Wakayama, Japan.
  • Tamura T; Department of Ophthalmology, Wakayama Medical University School of Medicine, Wakayama, Japan.
  • Yamanaka O; Department of Ophthalmology, Wakayama Medical University School of Medicine, Wakayama, Japan.
  • Saika S; Department of Ophthalmology, Wakayama Medical University School of Medicine, Wakayama, Japan.
Curr Eye Res ; : 1-8, 2024 Jul 21.
Article en En | MEDLINE | ID: mdl-39034645
ABSTRACT

PURPOSE:

Effect of topical administration of a Rho kinase inhibitor, ripasudil, on epithelial wound healing in a mouse cornea was investigated. Effects of treatment of cultured human corneal epithelial cell (HCEC) line and organ-cultured corneal epithelium with ripasudil on expression of p-ERK was also examined.

METHODS:

Epithelial defects with a diameter of 2.0 mm were prepared in the central corneas of C57BL/6 mice with or without 1-week travoprost pre-treatment, to which ripasudil or PBS as a control was instilled every 6 h immediately after preparation. The mice eyes were cultured with or without travoprost for 24-hrs. The expression levels of p-ERK in epithelium of mice eyes were compared by immunostaining after further 24-hrs culture with or without ripasudil for 24-hrs. HCEC were cultured with or without ripasudil and processed for examination for proliferation activity and protein expression of p-ERK by either immunostaining or Western blotting. The cells were also treated with or without travoprost for 24-hrs, and were further cultured with or without ripasudil. Expression levels of p-ERK were examined by Western blotting.

RESULTS:

Ripasudil treatment suppressed post-debridement epithelial healing in association with reduced proliferation activity in peripheral (limbal) epithelium in cornea with or without pre-treatment with travoprost. Ripasudil treatment accelerated p-ERK expression. Ripasudil supplementation upregulated proliferation with increased p-ERK in HCEC.

CONCLUSION:

Ripasudil treatment promotes wound healing of the mouse corneal epithelium by enhancing cell proliferation on peripheral (limbal) epithelium.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Curr Eye Res Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Curr Eye Res Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido