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Establishment and evaluation of a stable and reliable rat model of peritoneal adhesions.
Nian, Hongyu; Pu, Zhenjun; Li, Zhiyuan; Zhong, Pengfei; Ma, Shengxu; Li, Junliang.
Afiliación
  • Nian H; The First School of Clinical Medical, Gansu University of Chinese Medicine, Lanzhou, China; Department of General Surgery, Gansu Provincial Hospital, Lanzhou, China.
  • Pu Z; The First School of Clinical Medical, Gansu University of Chinese Medicine, Lanzhou, China; Department of General Surgery, Gansu Provincial Hospital, Lanzhou, China.
  • Li Z; The First School of Clinical Medical, Gansu University of Chinese Medicine, Lanzhou, China.
  • Zhong P; The First School of Clinical Medical, Gansu University of Chinese Medicine, Lanzhou, China.
  • Ma S; The First School of Clinical Medical, Gansu University of Chinese Medicine, Lanzhou, China.
  • Li J; The First School of Clinical Medical, Gansu University of Chinese Medicine, Lanzhou, China; Department of General Surgery, Gansu Provincial Hospital, Lanzhou, China; Key Laboratory of Molecular Diagnostics and Precision Medicine for Surgical Oncology in Gansu Province, Gansu Provincial Hospital, Lan
Surgery ; 176(4): 1256-1262, 2024 Oct.
Article en En | MEDLINE | ID: mdl-39034213
ABSTRACT

BACKGROUND:

In this study, we aimed to establish a stable and standardized animal model of peritoneal adhesions.

METHODS:

Forty-eight male Sprague-Dawley rats were randomly divided (n = 12 each) into blank control, classic cecum sidewall, ischemic button, and cecum-sidewall suture groups. The modified American Fertility Society adhesion score was used on postoperative day 7 to evaluate adhesions. Sixty male Sprague-Dawley rats were used to dynamically observe the adhesion characteristics of cecum-sidewall ischemic injury suture model at different time points (n = 60, randomly divided into groups a-e with 12 rats each). The modified American Fertility Society and Zühlke histologic scoring systems, hematoxylin-eosin staining, Masson staining, and computed tomography of the abdomen were used to evaluate adhesions on postoperative days 1, 3, 5, 7, and 14.

RESULTS:

No peritoneal adhesions were observed in the blank control group on postoperative day 7. In the classic cecum sidewall group, 8 rats had inconsistent adhesions, which had a modified American Fertility Society adhesion score of 2.25 ± 1.96. All rats in the ischemic button and cecum-sidewall suture groups developed significant adhesions with modified American Fertility Society scores of 3.08 ± 1.31 and 4.67 ± 0.78, respectively. When the modified American Fertility Society score was used, statistically significant differences were observed between the classic cecum sidewall groups and cecum-sidewall suture groups and between the ischemic button groups and cecum-sidewall suture groups. All animals in groups a-e developed adhesions; adhesion scores increased gradually with time.

CONCLUSIONS:

The cecum-sidewall ischemic injury suture model is a stable and standardized animal model of peritoneal adhesions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades Peritoneales / Ratas Sprague-Dawley / Modelos Animales de Enfermedad Límite: Animals Idioma: En Revista: Surgery Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades Peritoneales / Ratas Sprague-Dawley / Modelos Animales de Enfermedad Límite: Animals Idioma: En Revista: Surgery Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos