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Resistance to protease inhibitors among persons living with HIV in Ghana: a case for viral load and drug resistance monitoring.
Seshie, Makafui; Obeng, Billal Musah; Boamah, Vivian Etsiapa; Bayor, Marcel; Bonney, Evelyn Yayra; Gbedema, Stephen Yao; Sagoe, Kwamena William Coleman.
Afiliación
  • Seshie M; Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana. amaseshie@gmail.com.
  • Obeng BM; Department of Pharmaceutics, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana. amaseshie@gmail.com.
  • Boamah VE; Department of Medical Microbiology, School of Biomedical and Allied Health Sciences, University of Ghana, Accra, Ghana. vifredobeng@gmail.com.
  • Bayor M; Immunovirology and Pathogenesis Program, Kirby Institute, University of New South Wales, Sydney, Australia. vifredobeng@gmail.com.
  • Bonney EY; Department of Pharmaceutics, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
  • Gbedema SY; Department of Pharmaceutics, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
  • Sagoe KWC; Department of Virology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana.
Virol J ; 21(1): 159, 2024 Jul 20.
Article en En | MEDLINE | ID: mdl-39033275
ABSTRACT

OBJECTIVE:

Routine viral load and drug resistance testing are well supported in most resource-rich settings and provide valuable benefits in the clinical care of PLWH in these communities. Undoubtedly, there exist financial and political constraints for the scale-up of viral load and drug resistance testing in Sub-Saharan Africa. To achieve the global UNAIDS 95/95/95 targets, there is the need to bridge this inequity in patient care and allow for a universal approach that leaves no community behind.

METHODS:

Venous blood from 96 PLWH on second-line ART from Korle-Bu Teaching Hospital were collected and processed into plasma for CD4+ T- cell and viral load assessments. Ribonucleic acid (RNA) was extracted from stored plasma and the protease gene amplified, sequenced and analyzed for subtype and drug resistance mutations using the Stanford HIV drug resistance database.

RESULTS:

Out of the 96 PLWH, 37 experienced virological failure with 8 patients' samples successfully sequenced. The predominant HIV-1 subtype identified was CRF02_AG (6/8, 75.0%) with 12.5% (1/8) each of CFR06_cpx infection and one case unable to subtype. The major PI resistance mutations identified were; M46I, I54V, V82A, I47V, I84V and L90M.

CONCLUSIONS:

Persons living with HIV who had experienced virologic failure in this study harboured drug resistance mutations to PI, thus compromise the effectiveness of the drugs in the second line. Resistance testing is strongly recommended prior to switching to a new regimen. This will help to inform the choice of drug and to achieve optimum therapeutic outcome among PLWH in Ghana.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por VIH / VIH-1 / Inhibidores de la Proteasa del VIH / Carga Viral / Farmacorresistencia Viral Límite: Adult / Female / Humans / Male / Middle aged País/Región como asunto: Africa Idioma: En Revista: Virol J Asunto de la revista: VIROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Ghana Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por VIH / VIH-1 / Inhibidores de la Proteasa del VIH / Carga Viral / Farmacorresistencia Viral Límite: Adult / Female / Humans / Male / Middle aged País/Región como asunto: Africa Idioma: En Revista: Virol J Asunto de la revista: VIROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Ghana Pais de publicación: Reino Unido