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High-yield magnetosome production of Magnetospirillum magneticum strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation.
Wang, Yu; Liu, Zhengyi; Li, Wenjun; Cui, Hongli; Huang, Yandi; Qin, Song.
Afiliación
  • Wang Y; Key Laboratory of Coastal Biology and Biological Resource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, No. 17, Chunhui Road, Laishan District, Yantai, 264003, Shandong, China.
  • Liu Z; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Li W; Key Laboratory of Coastal Biology and Biological Resource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, No. 17, Chunhui Road, Laishan District, Yantai, 264003, Shandong, China.
  • Cui H; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Huang Y; Key Laboratory of Coastal Biology and Biological Resource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, No. 17, Chunhui Road, Laishan District, Yantai, 264003, Shandong, China.
  • Qin S; University of Chinese Academy of Sciences, Beijing, 100049, China.
Biotechnol Lett ; 2024 Jul 20.
Article en En | MEDLINE | ID: mdl-39031272
ABSTRACT

OBJECTIVES:

Developing a simplified flask fermentation strategy utilizing magnetotactic bacterium AMB-1 and optimized iron supplementation for high-yield magnetosome production to address the challenges associated with magnetosome acquisition.

RESULTS:

A reliable processing for the pure culture of AMB-1 was established using standard laboratory consumables and equipment. Subsequently, the medium and iron supplementation were optimized to enhance the yield of AMB-1 magnetosomes. The mSLM supported higher biomass accumulation in flask fermentation, reaching an OD565 of ~ 0.7. The premixed solution of ferric quinate and EDTA-Fe (at a ratio of 0.50.5 and a concentration of 0.4 mmol/L) stabilized Fe3+ and significantly increased the reductase activity of AMB-1. Flask fermentations with an initial volume of 15 L were then conducted employing the optimized fermentation strategy. After two rounds of iron and nutrient supplementation, the magnetosome yield reached 185.7 ± 9.5 mg/batch (approximately 12 mg/L), representing the highest AMB-1 flask fermentation yield to our knowledge.

CONCLUSION:

A flask fermentation strategy for high-yield magnetsome production was developed, eliminating the need for bioreactors and greatly simplifying the process of magnetosome acquisition.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biotechnol Lett Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biotechnol Lett Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos