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Recombinant 3AB3 nonstructural protein-based indirect ELISA for detection of foot-and-mouth disease virus infection-elicited antibodies in goat.
Rout, M; Pandey, L K; Prusty, B R; Samanta, R; Mohapatra, J K; Singh, R P.
Afiliación
  • Rout M; International Centre for Foot and Mouth Disease, ICAR-National Institute on Foot and Mouth Disease, Bhubaneswar, 752050, Odisha, India. drmrout@gmail.com.
  • Pandey LK; St. Aloysius College, Jabalpur, Madhya Pradesh, India.
  • Prusty BR; Animal Biotechnology Centre, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India.
  • Samanta R; International Centre for Foot and Mouth Disease, ICAR-National Institute on Foot and Mouth Disease, Bhubaneswar, 752050, Odisha, India.
  • Mohapatra JK; International Centre for Foot and Mouth Disease, ICAR-National Institute on Foot and Mouth Disease, Bhubaneswar, 752050, Odisha, India.
  • Singh RP; International Centre for Foot and Mouth Disease, ICAR-National Institute on Foot and Mouth Disease, Bhubaneswar, 752050, Odisha, India.
Vet Res Commun ; 2024 Jul 20.
Article en En | MEDLINE | ID: mdl-39031217
ABSTRACT
Foot-and-mouth disease (FMD) is one of the most important animal diseases of economic significance globally. It is a highly infectious and contagious disease of cloven-hoofed animals including sheep and goat. For sero-diagnosis of FMD, recombinant antigen-based assays are considered as alternatives to conventional approaches such as the liquid phase blocking ELISA (LPBE). The early interventions towards control measures cannot be implemented unless the disease gets promptly diagnosed. It is relatively difficult to clinically diagnose FMD in goat due to the usual milder form or unapparent nature of symptoms. Under such situations where clinical samples are not available, demonstration of infection-specific FMD virus (FMDV) antibodies in serum sample may help identifying the animals exposed to the virus in retrospect. Antibody to 3AB nonstructural protein (NSP) has been considered to be the most reliable indicator for FMD diagnosis. The current study extended the earlier designed recombinant 3AB3 protein-based indirect ELISA originally validated on bovine serum samples to testing serum samples of goat. The performance of the indirect ELISA was validated using internationally accepted PrioCHECK® FMDV NS kit. The overall diagnostic sensitivity (DSn) of the indirect ELISA was estimated to be 95.52% (619/648), while the diagnostic specificity (DSp) on naïve and vaccinated animals varied at 98.06% (557/568) and 94.15% (435/462), respectively. In India, where FMD is prevalent and the goat population is so high, this 'in-house' optimized assay can be considered to be an adjunct in sero-epidemiological investigation of FMD in goat.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vet Res Commun Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vet Res Commun Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Suiza