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Osmoregulation in freshwater anaerobic methane-oxidizing archaea under salt stress.
Echeveste Medrano, Maider J; Leu, Andy O; Pabst, Martin; Lin, Yuemei; McIlroy, Simon J; Tyson, Gene W; van Ede, Jitske; Sánchez-Andrea, Irene; Jetten, Mike S M; Jansen, Robert; Welte, Cornelia U.
Afiliación
  • Echeveste Medrano MJ; Department of Microbiology, Radboud Institute for Biological and Environmental Sciences (RIBES), Radboud University, Heyendaalseweg 135, 6525AJ Nijmegen, The Netherlands.
  • Leu AO; Centre for Microbiome Research (CMR), School of Biomedical Sciences, Queensland University of Technology (QUT), Translational Research Institute (TRI), 37 Kent Street, Woolloongabba, QLD 4102, Australia.
  • Pabst M; Department of Environmental Biotechnology, TU-Delft University, Van der Maasweg 9, 2629HZ Delft, The Netherlands.
  • Lin Y; Department of Environmental Biotechnology, TU-Delft University, Van der Maasweg 9, 2629HZ Delft, The Netherlands.
  • McIlroy SJ; Centre for Microbiome Research (CMR), School of Biomedical Sciences, Queensland University of Technology (QUT), Translational Research Institute (TRI), 37 Kent Street, Woolloongabba, QLD 4102, Australia.
  • Tyson GW; Centre for Microbiome Research (CMR), School of Biomedical Sciences, Queensland University of Technology (QUT), Translational Research Institute (TRI), 37 Kent Street, Woolloongabba, QLD 4102, Australia.
  • van Ede J; Department of Environmental Biotechnology, TU-Delft University, Van der Maasweg 9, 2629HZ Delft, The Netherlands.
  • Sánchez-Andrea I; Department of Environmental Sciences for Sustainability, IE University, C. Cardenal Zúñiga 12, 40003 Segovia, Spain.
  • Jetten MSM; Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708WE Wageningen, The Netherlands.
  • Jansen R; Department of Microbiology, Radboud Institute for Biological and Environmental Sciences (RIBES), Radboud University, Heyendaalseweg 135, 6525AJ Nijmegen, The Netherlands.
  • Welte CU; Department of Microbiology, Radboud Institute for Biological and Environmental Sciences (RIBES), Radboud University, Heyendaalseweg 135, 6525AJ Nijmegen, The Netherlands.
ISME J ; 18(1)2024 Jan 08.
Article en En | MEDLINE | ID: mdl-39030685
ABSTRACT
Climate change-driven sea level rise threatens freshwater ecosystems and elicits salinity stress in microbiomes. Methane emissions in these systems are largely mitigated by methane-oxidizing microorganisms. Here, we characterized the physiological and metabolic response of freshwater methanotrophic archaea to salt stress. In our microcosm experiments, inhibition of methanotrophic archaea started at 1%. However, during gradual increase of salt up to 3% in a reactor over 12 weeks, the culture continued to oxidize methane. Using gene expression profiles and metabolomics, we identified a pathway for salt-stress response that produces the osmolyte of anaerobic methanotrophic archaea N(ε)-acetyl-ß-L-lysine. An extensive phylogenomic analysis on N(ε)-acetyl-ß-L-lysine-producing enzymes revealed that they are widespread across both bacteria and archaea, indicating a potential horizontal gene transfer and a link to BORG extrachromosomal elements. Physicochemical analysis of bioreactor biomass further indicated the presence of sialic acids and the consumption of intracellular polyhydroxyalkanoates in anaerobic methanotrophs during salt stress.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Archaea / Osmorregulación / Agua Dulce / Estrés Salino / Metano Idioma: En Revista: ISME J Asunto de la revista: MICROBIOLOGIA / SAUDE AMBIENTAL Año: 2024 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Archaea / Osmorregulación / Agua Dulce / Estrés Salino / Metano Idioma: En Revista: ISME J Asunto de la revista: MICROBIOLOGIA / SAUDE AMBIENTAL Año: 2024 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido