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Combinatorial strain improvement and bioprocess development for efficient production of ε-poly-L-lysine in Streptomyces albulus.
Zhu, Daojun; Liu, Yuxiang; Yang, Hao; Zhang, Jiawei; Zheng, Gencheng; Zhang, Hongjian; Wang, Liang; Zhang, Jianhua; Chen, Xusheng.
Afiliación
  • Zhu D; Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
  • Liu Y; Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
  • Yang H; Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
  • Zhang J; Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
  • Zheng G; Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
  • Zhang H; Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
  • Wang L; Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
  • Zhang J; Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
  • Chen X; Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: chenxs@jiangnan.edu.cn.
Bioresour Technol ; 407: 131123, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39029766
ABSTRACT
ε-Poly-L-lysine (ε-PL) is an amino acid homopolymer with diverse potential applications in the food, pharmaceutical and cosmetic industries. To improve its biomanufacturing efficiency, strain engineering and bioprocess optimization were combined in this study. Firstly, a cocktail strain breeding strategy was employed to generate a ε-PL high-production mutant, Streptomyces albulus GS114, with enhanced L-lysine uptake capability. Subsequently, the L-lysine feeding conditions during fed-batch fermentation were systematically optimized to improve the L-lysine supply, resulting in ε-PL production reaching 73.1 ± 1.4 g/L in 5 L bioreactor. Finally, an engineered strain, S. albulus L2, with enhanced uptake capability and polymerization ability of L-lysine was constructed, achieving ε-PL production of 81.4 ± 5.2 g/L by fed-batch fermentation. This represents the highest reported production of ε-PL to date. This study provided an efficient production strategy for ε-PL and valuable insights into the high-value utilization of L-lysine.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polilisina / Streptomyces / Reactores Biológicos / Fermentación / Lisina Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polilisina / Streptomyces / Reactores Biológicos / Fermentación / Lisina Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido