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Somatic CpG hypermutation is associated with mismatch repair deficiency in cancer.
Flynn, Aidan; Waszak, Sebastian M; Weischenfeldt, Joachim.
Afiliación
  • Flynn A; Biotech Research & Innovation Centre (BRIC), University of Copenhagen, Copenhagen, Denmark.
  • Waszak SM; The Finsen Laboratory, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark.
  • Weischenfeldt J; Department of Clinical Pathology and Centre for Cancer Research, University of Melbourne, Parkville, VIC, Australia.
Mol Syst Biol ; 20(9): 1006-1024, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39026103
ABSTRACT
Somatic hypermutation in cancer has gained momentum with the increased use of tumour mutation burden as a biomarker for immune checkpoint inhibitors. Spontaneous deamination of 5-methylcytosine to thymine at CpG dinucleotides is one of the most ubiquitous endogenous mutational processes in normal and cancer cells. Here, we performed a systematic investigation of somatic CpG hypermutation at a pan-cancer level. We studied 30,191 cancer patients and 103 cancer types and developed an algorithm to identify somatic CpG hypermutation. Across cancer types, we observed the highest prevalence in paediatric leukaemia (3.5%), paediatric high-grade glioma (1.7%), and colorectal cancer (1%). We discovered germline variants and somatic mutations in the mismatch repair complex MutSα (MSH2-MSH6) as genetic drivers of somatic CpG hypermutation in cancer, which frequently converged on CpG sites and TP53 driver mutations. We further observe an association between somatic CpG hypermutation and response to immune checkpoint inhibitors. Overall, our study identified novel cancer types that display somatic CpG hypermutation, strong association with MutSα-deficiency, and potential utility in cancer immunotherapy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Islas de CpG / Reparación de la Incompatibilidad de ADN / Mutación / Neoplasias Límite: Humans Idioma: En Revista: Mol Syst Biol Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Islas de CpG / Reparación de la Incompatibilidad de ADN / Mutación / Neoplasias Límite: Humans Idioma: En Revista: Mol Syst Biol Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Alemania