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3D printed rectal swabs for assessing the gut microbiome, metabolome and inflammation.
Perry, Robert W; Mullish, Benjamin H; Alexander, James L; Shah, Raashi; Danckert, Nathan P; Blanco, Jesus Miguens; Roberts, Lauren; Liu, Zhigang; Chrysostomou, Despoina; Radhakrishnan, Shiva T; Balarajah, Sharmili; Barry, Rachael; Hicks, Lucy C; Williams, Horace R T; Marchesi, Julian R.
Afiliación
  • Perry RW; Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London, UK. robert.perry@imperial.ac.uk.
  • Mullish BH; Departments of Gastroenterology and Hepatology, St Mary's Hospital, Imperial College Healthcare NHS Trust, London, UK. robert.perry@imperial.ac.uk.
  • Alexander JL; Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London, UK.
  • Shah R; Departments of Gastroenterology and Hepatology, St Mary's Hospital, Imperial College Healthcare NHS Trust, London, UK.
  • Danckert NP; Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London, UK.
  • Blanco JM; Departments of Gastroenterology and Hepatology, St Mary's Hospital, Imperial College Healthcare NHS Trust, London, UK.
  • Roberts L; Department of Gastroenterology, St Marks Hospital, London, UK.
  • Liu Z; Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London, UK.
  • Chrysostomou D; Department of Twin Research and Genetic Epidemiology, School of Life Course & Population Sciences, King's College London, London, UK.
  • Radhakrishnan ST; Department of Bioengineering, Stanford University, Stanford, CA, USA.
  • Balarajah S; Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London, UK.
  • Barry R; Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London, UK.
  • Hicks LC; Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London, UK.
  • Williams HRT; Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London, UK.
  • Marchesi JR; Department of Gastroenterology, St Marks Hospital, London, UK.
Sci Rep ; 14(1): 16613, 2024 07 18.
Article en En | MEDLINE | ID: mdl-39026025
ABSTRACT
Investigating the gut microbiome and metabolome frequently requires faecal samples, which can be difficult to obtain. Previous studies have shown that rectal swabs are comparable to faecal samples for analysing gut microbiota composition and key metabolites. In this study, 3D printed rectal swabs were compared with conventional flocked swabs and faecal samples, due to the potential advantages 3D printing as a technique offers for swab production and development. 16S rRNA gene sequencing, qPCR and metabolite profiling (using 1H-NMR spectroscopy) were performed on swab and faecal samples from healthy participants. Faecal calprotectin and total protein analysis were performed on samples from inflammatory bowel disease (IBD) patients. There were no significant differences between both swab types and faecal samples when assessing key measures of alpha and beta diversity, and differences in the abundance of major phyla. There was a strong correlation between both swab types and faecal samples for all combined metabolites detected by NMR. In IBD patients, there was no significant difference in faecal calprotectin and total protein levels between both swab types and faecal samples. These data lead us to conclude that 3D printed swabs are equivalent to flocked swabs for the analysis of the gut microbiome, metabolome and inflammation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Ribosómico 16S / Enfermedades Inflamatorias del Intestino / Metaboloma / Heces / Impresión Tridimensional / Microbioma Gastrointestinal Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Ribosómico 16S / Enfermedades Inflamatorias del Intestino / Metaboloma / Heces / Impresión Tridimensional / Microbioma Gastrointestinal Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido