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Enhancing Rose Bengal penetration in ex vivo human corneas using iontophoresis.
Lai, James M; Chen, Justin; Navia, Juan Carlos; Durkee, Heather; Gonzalez, Alex; Rowaan, Cornelis; Arcari, Timothy; Aguilar, Mariela C; Llanes, Katrina; Ziebarth, Noel; Martinez, Jaime D; Miller, Darlene; Flynn, Harry W; Amescua, Guillermo; Parel, Jean-Marie.
Afiliación
  • Lai JM; Ophthalmic Biophysics Center, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Chen J; Ophthalmic Biophysics Center, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Navia JC; Ophthalmic Biophysics Center, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Durkee H; Ophthalmic Biophysics Center, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Gonzalez A; Ophthalmic Biophysics Center, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Rowaan C; Ophthalmic Biophysics Center, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Arcari T; Ophthalmic Biophysics Center, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Aguilar MC; Department of Biomedical Engineering, University of Miami, Coral Gables, FL 33146, USA.
  • Llanes K; Ophthalmic Biophysics Center, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Ziebarth N; Florida Lions Eye Bank, Miami, FL  33136, USA.
  • Martinez JD; Department of Biomedical Engineering, University of Miami, Coral Gables, FL 33146, USA.
  • Miller D; Anne Bates Leach Eye Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Flynn HW; Anne Bates Leach Eye Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
  • Amescua G; Ocular Microbiology Laboratory, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, FL  33136, USA.
  • Parel JM; Anne Bates Leach Eye Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Ther Deliv ; 15(8): 567-575, 2024.
Article en En | MEDLINE | ID: mdl-39023301
ABSTRACT

Aim:

Rose Bengal photodynamic antimicrobial therapy (RB-PDAT) has poor corneal penetration, limiting its efficacy against acanthamoeba keratitis (AK). Iontophoresis enhances corneal permeation of charged molecules, piquing interest in its effects on RB in ex vivo human corneas.

Methods:

Five donor whole globes each underwent iontophoresis with RB, soaking in RB, or were soaked in normal saline (controls). RB penetration and corneal thickness was assessed using confocal microscopy.

Results:

Iontophoresis increased RB penetration compared with soaking (177 ± 9.5 µm vs. 100 ± 5.7 µm, p < 0.001), with no significant differences in corneal thickness between groups (460 ± 87 µm vs. 407 ± 69 µm, p = 0.432).

Conclusion:

Iontophoresis significantly improves RB penetration and its use in PDAT could offer a novel therapy for acanthamoeba keratitis. Further studies are needed to validate clinical efficacy.
The study aimed to improve a new treatment for eye infections known as photodynamic antimicrobial therapy. It investigated whether the use of electricity through a technique called iontophoresis could help a chemical called Rose Bengal go deeper into the eye in order to target more severe infections. The iontophoresis machine was custom built, with patient-contacting components 3D printed. The experiments were performed using donated human eye tissue and found that iontophoresis significantly improved the penetration depth of Rose Bengal as compared with the current technique of only soaking the eye in Rose Bengal.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rosa Bengala / Queratitis por Acanthamoeba / Iontoforesis / Córnea Límite: Humans Idioma: En Revista: Ther Deliv Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rosa Bengala / Queratitis por Acanthamoeba / Iontoforesis / Córnea Límite: Humans Idioma: En Revista: Ther Deliv Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido