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scMicrobe PTA: near complete genomes from single bacterial cells.
Bowers, Robert M; Gonzalez-Pena, Veronica; Wardhani, Kartika; Goudeau, Danielle; Blow, Matthew James; Udwary, Daniel; Klein, David; Vill, Albert C; Brito, Ilana L; Woyke, Tanja; Malmstrom, Rex R; Gawad, Charles.
Afiliación
  • Bowers RM; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, United States.
  • Gonzalez-Pena V; Department of Pediatrics, Stanford University, Stanford, CA, United States.
  • Wardhani K; Department of Pediatrics, Stanford University, Stanford, CA, United States.
  • Goudeau D; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, United States.
  • Blow MJ; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, United States.
  • Udwary D; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, United States.
  • Klein D; Department of Pediatrics, Stanford University, Stanford, CA, United States.
  • Vill AC; Chan Zuckerberg Biohub, San Francisco, CA, United States.
  • Brito IL; Chan Zuckerberg Biohub, San Francisco, CA, United States.
  • Woyke T; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, United States.
  • Malmstrom RR; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, United States.
  • Gawad C; Department of Pediatrics, Stanford University, Stanford, CA, United States.
ISME Commun ; 4(1): ycae085, 2024 Jan.
Article en En | MEDLINE | ID: mdl-39021442
ABSTRACT
Microbial genomes produced by standard single-cell amplification methods are largely incomplete. Here, we show that primary template-directed amplification (PTA), a novel single-cell amplification technique, generated nearly complete genomes from three bacterial isolate species. Furthermore, taxonomically diverse genomes recovered from aquatic and soil microbiomes using PTA had a median completeness of 81%, whereas genomes from standard multiple displacement amplification-based approaches were usually <30% complete. PTA-derived genomes also included more associated viruses and biosynthetic gene clusters.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ISME Commun Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ISME Commun Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido