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Morphotype-specific calcium signaling in human microglia.
Nevelchuk, Sofia; Brawek, Bianca; Schwarz, Niklas; Valiente-Gabioud, Ariel; Wuttke, Thomas V; Kovalchuk, Yury; Koch, Henner; Höllig, Anke; Steiner, Frederik; Figarella, Katherine; Griesbeck, Oliver; Garaschuk, Olga.
Afiliación
  • Nevelchuk S; Department of Neurophysiology, Institute of Physiology, Eberhard Karls University of Tübingen, Keplerstr. 15, 72074, Tübingen, Germany.
  • Brawek B; Department of Neurophysiology, Institute of Physiology, Eberhard Karls University of Tübingen, Keplerstr. 15, 72074, Tübingen, Germany.
  • Schwarz N; Department of Neurology and Epileptology, Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany.
  • Valiente-Gabioud A; Tools for Bio-Imaging, Max-Planck-Institute for Biological Intelligence, Martinsried, Germany.
  • Wuttke TV; Department of Neurology and Epileptology, Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany.
  • Kovalchuk Y; Department of Neurosurgery, University of Tübingen, Tübingen, Germany.
  • Koch H; Department of Neurophysiology, Institute of Physiology, Eberhard Karls University of Tübingen, Keplerstr. 15, 72074, Tübingen, Germany.
  • Höllig A; Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany.
  • Steiner F; Department of Neurosurgery, RWTH Aachen University, Aachen, Germany.
  • Figarella K; Department of Neurophysiology, Institute of Physiology, Eberhard Karls University of Tübingen, Keplerstr. 15, 72074, Tübingen, Germany.
  • Griesbeck O; Department of Neurophysiology, Institute of Physiology, Eberhard Karls University of Tübingen, Keplerstr. 15, 72074, Tübingen, Germany.
  • Garaschuk O; Department of Anesthesiology, Critical Care and Pain Medicine, University of Texas Health Science Center at Houston, Houston, TX, USA.
J Neuroinflammation ; 21(1): 175, 2024 Jul 17.
Article en En | MEDLINE | ID: mdl-39020359
ABSTRACT

BACKGROUND:

Key functions of Ca2+ signaling in rodent microglia include monitoring the brain state as well as the surrounding neuronal activity and sensing the danger or damage in their vicinity. Microglial Ca2+ dyshomeostasis is a disease hallmark in many mouse models of neurological disorders but the Ca2+ signal properties of human microglia remain unknown.

METHODS:

We developed a novel genetically-encoded ratiometric Ca2+ indicator, targeting microglial cells in the freshly resected human tissue, organotypically cultured tissue slices and analyzed in situ ongoing Ca2+ signaling of decades-old microglia dwelling in their native microenvironment.

RESULTS:

The data revealed marked compartmentalization of Ca2+ signals, with signal properties differing across the compartments and resident morphotypes. The basal Ca2+ levels were low in ramified and high in ameboid microglia. The fraction of cells with ongoing Ca2+ signaling, the fraction and the amplitude of process Ca2+ signals and the duration of somatic Ca2+ signals decreased when moving from ramified via hypertrophic to ameboid microglia. In contrast, the size of active compartments, the fraction and amplitude of somatic Ca2+ signals and the duration of process Ca2+ signals increased along this pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Microglía / Señalización del Calcio Límite: Female / Humans / Male Idioma: En Revista: J Neuroinflammation Asunto de la revista: NEUROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcio / Microglía / Señalización del Calcio Límite: Female / Humans / Male Idioma: En Revista: J Neuroinflammation Asunto de la revista: NEUROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido