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Extracellular Matrix Protein Signatures of the Outer and Inner Zones of the Rat Adrenal Cortex.
Kremer, Jean Lucas; Santiago, Veronica Feijoli; Bongiovani Rodrigues, Fernanda; Auricino, Thais Barabba; Freitas, Danilo Henriques de Oliveira; Palmisano, Giuseppe; Lotfi, Claudimara Ferini Pacicco.
Afiliación
  • Kremer JL; Institute of Biomedical Sciences, Department of Anatomy, University of São Paulo, Av. Prof. Lineu Prestes, 2415, Butantã, São Paulo, SP 05508-000, Brazil.
  • Santiago VF; Institute of Biomedical Sciences, Department of Parasitology, University of São Paulo, Av. Prof. Lineu Prestes, 1374, Butantã, São Paulo, SP 05508-000, Brazil.
  • Bongiovani Rodrigues F; Institute of Biomedical Sciences, Department of Anatomy, University of São Paulo, Av. Prof. Lineu Prestes, 2415, Butantã, São Paulo, SP 05508-000, Brazil.
  • Auricino TB; Institute of Biomedical Sciences, Department of Anatomy, University of São Paulo, Av. Prof. Lineu Prestes, 2415, Butantã, São Paulo, SP 05508-000, Brazil.
  • Freitas DHO; Institute of Biomedical Sciences, Department of Anatomy, University of São Paulo, Av. Prof. Lineu Prestes, 2415, Butantã, São Paulo, SP 05508-000, Brazil.
  • Palmisano G; Institute of Biomedical Sciences, Department of Parasitology, University of São Paulo, Av. Prof. Lineu Prestes, 1374, Butantã, São Paulo, SP 05508-000, Brazil.
  • Lotfi CFP; Institute of Biomedical Sciences, Department of Anatomy, University of São Paulo, Av. Prof. Lineu Prestes, 2415, Butantã, São Paulo, SP 05508-000, Brazil.
J Proteome Res ; 23(8): 3418-3432, 2024 Aug 02.
Article en En | MEDLINE | ID: mdl-39018382
ABSTRACT
This study analyzes the extracellular matrix (ECM) signatures of the outer (OF = capsule + subcapsular + zona glomerulosa cells) and inner fractions (IF = zona fasciculata cells) of the rat adrenal cortex, which comprise two distinct microenvironment niches. Proteomic profiles of decellularized OF and IF samples, male and female rats, identified 252 proteins, with 32 classified as ECM-component and ECM-related. Among these, 25 proteins were differentially regulated 17 more abundant in OF, including Col1a1, Col1a2, Col6a1, Col6a2, Col6a3, Col12a1, Col14a1, Lama5, Lamb2, Lamc1, Eln, Emilin, Fbln5, Fbn1, Fbn2, Nid1, and Ltbp4, and eight more abundant in IF, including Col4a1, Col4a2, Lama2, Lama4, Lamb1, Fn1, Hspg2, and Ecm1. Eln, Tnc, and Nid2 were abundant in the female OF, while Lama2, Lama5, Lamb2, and Lamc1 were more abundant in the male IF. The complex protein signature of the OF suggests areas of tissue stress, stiffness, and regulatory proteins for growth factor signaling. The higher concentrations of Col4a1 and Col4a2 and their role in steroidogenesis should be further investigated in IF. These findings could significantly enhance our understanding of adrenal cortex functionality and its implications for human health and disease. Key findings were validated, and data are available in ProteomeXchange (PXD046828).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de la Matriz Extracelular / Corteza Suprarrenal Límite: Animals Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de la Matriz Extracelular / Corteza Suprarrenal Límite: Animals Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos