Your browser doesn't support javascript.
loading
Visualizing Epigenetic Modifications and Nuclear Bodies by Immunofluorescence Staining in Naïve, Activated, and Memory B Cell Subsets.
Chakma, Clarissa R; Garvie, Andrew; Wong, Lee H; Good-Jacobson, Kim L.
Afiliación
  • Chakma CR; Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, Australia.
  • Garvie A; Immunity Program, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
  • Wong LH; Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, Australia.
  • Good-Jacobson KL; Cancer Program, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
Methods Mol Biol ; 2826: 47-54, 2024.
Article en En | MEDLINE | ID: mdl-39017884
ABSTRACT
Immunofluorescence microscopy is a powerful technique using fluorescently labelled antibodies which can be used to visualize proteins in the nucleus. A key advantage of this method is that it can provide insight into the spatial organization and the localization of nuclear proteins, which can provide elucidation of their function. Here, we provide a protocol for immunofluorescence staining in the nucleus, which has successfully been used to visualize histone modifications and nuclear bodies in human and mouse B lymphocytes, using as few as 1 × 104-5 × 104 cells.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnica del Anticuerpo Fluorescente / Epigénesis Genética Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnica del Anticuerpo Fluorescente / Epigénesis Genética Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos