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Evaluation of unmodified human cell-derived extracellular vesicle mitochondrial deoxyribonucleic acid-based biodistribution in rodents.
Cho, Young-Woo; Cho, Mi Young; Yoon, Jaehyeon; Hong, Da Eun; Lee, Ju-Young; Park, Hye Sun; Lee, Hyunseung; Hong, Kwan Soo; Won-Kyu, Lee; Saehae, Choi; Song, Suk-Gil; Noh, Young-Woock.
Afiliación
  • Cho YW; Division of Drug Safety Evaluation, NDDC, Osong Medical Innovation Foundation, Cheongju, South Korea.
  • Cho MY; College of Pharmacy, Chungbuk National University, Cheongju, South Korea.
  • Yoon J; Biopharmaceutical Research Center, Korea Basic Science Institute, Cheongju, South Korea.
  • Hong DE; Division of Drug Safety Evaluation, NDDC, Osong Medical Innovation Foundation, Cheongju, South Korea.
  • Lee JY; Division of Drug Safety Evaluation, NDDC, Osong Medical Innovation Foundation, Cheongju, South Korea.
  • Park HS; Division of Drug Safety Evaluation, NDDC, Osong Medical Innovation Foundation, Cheongju, South Korea.
  • Lee H; College of Pharmacy, Chungbuk National University, Cheongju, South Korea.
  • Hong KS; Biopharmaceutical Research Center, Korea Basic Science Institute, Cheongju, South Korea.
  • Won-Kyu L; Biopharmaceutical Research Center, Korea Basic Science Institute, Cheongju, South Korea.
  • Saehae C; Biopharmaceutical Research Center, Korea Basic Science Institute, Cheongju, South Korea.
  • Song SG; Department of Chemistry, Chung-Ang University, Seoul, South Korea.
  • Noh YW; Division of Drug Safety Evaluation, NDDC, Osong Medical Innovation Foundation, Cheongju, South Korea.
J Extracell Vesicles ; 13(7): e12489, 2024 Jul.
Article en En | MEDLINE | ID: mdl-39016198
ABSTRACT
Recently, extracellular vesicles (EVs) have been developed as therapeutic targets for various diseases. Biodistribution is crucial for EVs intended for therapeutic purposes because it can determine the degree of on- and off-target effects. This study aimed to explore techniques to evaluate the biodistribution of unmodified EVs. We devised a novel quantitative polymerase chain reaction (qPCR)-based assay to detect unmodified EVs by targeting mitochondrial deoxyribonucleic acid (mtDNA), a constituent of EVs. We focused on specific mtDNA regions that exhibited homologous variations distinct from their rodent mtDNA counterparts to establish this analytical approach. Herein, we successfully designed primers and probes targeting human and rodent mtDNA sequences and developed a highly specific and sensitive qPCR method. Furthermore, the quantification range of EVs isolated from various cells differed based on the manufacturer and cell source. IRDye 800CW-labelled Expi293F EV mimetics were administered to the animals via the tail vein to compare the imaging test and mtDNA-qPCR results. The results obtained from imaging tests and mtDNA-qPCR to investigate EV biodistribution patterns revealed differences. The results revealed that our newly developed method effectively determined the biodistribution of unmodified EVs with high sensitivity and reproducibility.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Mitocondrial / Vesículas Extracelulares Límite: Animals / Humans Idioma: En Revista: J Extracell Vesicles Año: 2024 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Mitocondrial / Vesículas Extracelulares Límite: Animals / Humans Idioma: En Revista: J Extracell Vesicles Año: 2024 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Estados Unidos