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Three-dimensional lung parenchyma model for studies of Aspergillus fumigatus infection and antifungal treatment.
Fortes, Bruna Nakanishi; Wirth, Fernanda; Dos Santos, Aline Martins; Chorilli, Marlus; Freitas, Vanessa Morais; Farias, Jennifer; Chambergo, Felipe S; Nunes C Dantas, Viviane Abreu; Ishida, Kelly.
Afiliación
  • Fortes BN; Institute of Biomedical Sciences, University of São Paulo, Prof. Lineu Prestes Avenue, 1374, 05508-000, São Paulo, Brazil.
  • Wirth F; Institute of Biomedical Sciences, University of São Paulo, Prof. Lineu Prestes Avenue, 1374, 05508-000, São Paulo, Brazil.
  • Dos Santos AM; School of Pharmaceutical Sciences, São Paulo State University - Jaú Highway, Km 1, 14800-903, Araraquara, Brazil.
  • Chorilli M; School of Pharmaceutical Sciences, São Paulo State University - Jaú Highway, Km 1, 14800-903, Araraquara, Brazil.
  • Freitas VM; Institute of Biomedical Sciences, University of São Paulo, Prof. Lineu Prestes Avenue, 1374, 05508-000, São Paulo, Brazil.
  • Farias J; School of Arts, Sciences & Humanities, University of São Paulo, Arlindo Bettio Avenue, 1000, 03828-000, São Paulo, Brazil.
  • Chambergo FS; School of Arts, Sciences & Humanities, University of São Paulo, Arlindo Bettio Avenue, 1000, 03828-000, São Paulo, Brazil.
  • Nunes C Dantas VA; School of Arts, Sciences & Humanities, University of São Paulo, Arlindo Bettio Avenue, 1000, 03828-000, São Paulo, Brazil.
  • Ishida K; Institute of Biomedical Sciences, University of São Paulo, Prof. Lineu Prestes Avenue, 1374, 05508-000, São Paulo, Brazil.
Future Microbiol ; : 1-14, 2024 Jul 16.
Article en En | MEDLINE | ID: mdl-39011856
ABSTRACT

Aim:

This work aims to standardize the three-dimensional hydroxyethyl-alginate-gelatin (HAG) scaffold as a model to evaluate Aspergillus fumigatus biofilm and antifungal treatments.

Methods:

The scaffold was characterized by physical, rheological and microscopic analyses; the antibiofilm action was evaluated by determination of cfu and metabolic activity.

Results:

The scaffold was non-toxic showing stability in aqueous media, swelling capacity, elasticity and had homogeneously distributed pores averaging 190 µm. The A. fumigatus biofilm established itself very well on the scaffold and treatment with amphotericin B and voriconazole reduced viable cells and metabolic activity.

Conclusion:

The HAG scaffold proved to be a model to mimic lung parenchyma, suitable for establishing a 3D biofilm culture of A. fumigatus and evaluating the efficacy of antifungals.
[Box see text].
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Future Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Future Microbiol Asunto de la revista: MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido