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Pulsed-field ablation: an alternative ablative method for gastric electrophysiological intervention.
Matthee, Ashton; Aghababaie, Zahra; Nisbet, Linley A; Dowrick, Jarrah M; Windsor, John A; Sands, Gregory B; Angeli-Gordon, Timothy R.
Afiliación
  • Matthee A; Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand.
  • Aghababaie Z; Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand.
  • Nisbet LA; Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand.
  • Dowrick JM; Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand.
  • Windsor JA; Department of Surgery, University of Auckland, Auckland, New Zealand.
  • Sands GB; Surgical and Translational Research Centre, University of Auckland, Auckland, New Zealand.
  • Angeli-Gordon TR; Auckland Bioengineering Institute, University of Auckland, Auckland, New Zealand.
Am J Physiol Gastrointest Liver Physiol ; 327(3): G456-G465, 2024 Sep 01.
Article en En | MEDLINE | ID: mdl-39010831
ABSTRACT
Pulsed-field ablation (PFA) is an emerging ablative technology that has been used successfully to eliminate cardiac arrhythmias. As a nonthermal technique, it has significant benefits over traditional radiofrequency ablation with improved target tissue specificity and reduced risk of adverse events during cardiac applications. We investigated whether PFA is safe for use in the stomach and whether it could modulate gastric slow waves. Female weaner pigs were fasted overnight before anesthesia was induced using tiletamine hydrochloride (50 mg·mL-1) and zolazepam hydrochloride (50 mg·mL-1) and maintained with propofol (Diprivan 2%, 0.2-0.4 mg·kg-1·min-1). Pulsed-field ablation was performed on their gastric serosa in vivo. Adjacent point lesions (n = 2-4) were used to create a linear injury using bipolar pulsed-field ablation consisting of 40 pulses (10 Hz frequency, 0.1 ms pulse width, 1,000 V amplitude). High-resolution electrical mapping defined baseline and postablation gastric slow-wave patterns. A validated five-point scale was used to evaluate tissue damage in hematoxylin and eosin-stained images. Results indicated that PFA successfully induced complete conduction blocks in all cases, with lesions through the entire thickness of the gastric muscle layers. Consistent postablation slow-wave patterns emerged immediately following ablation and persisted over the study period. Pulsed-field ablation induces rapid conduction blocks as a tool to modulate slow-wave patterns, indicating it may be suitable as an alternative to radiofrequency ablation.NEW & NOTEWORTHY Results show that pulsed-field ablation can serve as a gastric slow-wave intervention by preventing slow-wave propagation across the lesion site. Stable conduction blocks were established immediately following energy delivery, faster than previous examples of radiofrequency gastric ablation. Pulsed-field ablation may be an alternative for gastric slow-wave intervention, and further functional and posthealing studies are now warranted.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estómago Límite: Animals Idioma: En Revista: Am J Physiol Gastrointest Liver Physiol Asunto de la revista: FISIOLOGIA / GASTROENTEROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estómago Límite: Animals Idioma: En Revista: Am J Physiol Gastrointest Liver Physiol Asunto de la revista: FISIOLOGIA / GASTROENTEROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Estados Unidos