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Building a human lung from pluripotent stem cells to model respiratory viral infections.
Turner, Declan L; Amoozadeh, Sahel; Baric, Hannah; Stanley, Ed; Werder, Rhiannon B.
Afiliación
  • Turner DL; Murdoch Children's Research Institute, Melbourne, 3056, Australia.
  • Amoozadeh S; Department of Paediatrics, University of Melbourne, Melbourne, 3056, Australia.
  • Baric H; Novo Nordisk Foundation Centre for Stem Cell Medicine, reNEW Melbourne, Melbourne, 3056, Australia.
  • Stanley E; Murdoch Children's Research Institute, Melbourne, 3056, Australia.
  • Werder RB; Department of Paediatrics, University of Melbourne, Melbourne, 3056, Australia.
Respir Res ; 25(1): 277, 2024 Jul 15.
Article en En | MEDLINE | ID: mdl-39010108
ABSTRACT
To protect against the constant threat of inhaled pathogens, the lung is equipped with cellular defenders. In coordination with resident and recruited immune cells, this defence is initiated by the airway and alveolar epithelium following their infection with respiratory viruses. Further support for viral clearance and infection resolution is provided by adjacent endothelial and stromal cells. However, even with these defence mechanisms, respiratory viral infections are a significant global health concern, causing substantial morbidity, socioeconomic losses, and mortality, underlining the need to develop effective vaccines and antiviral medications. In turn, the identification of new treatment options for respiratory infections is critically dependent on the availability of tractable in vitro experimental models that faithfully recapitulate key aspects of lung physiology. For such models to be informative, it is important these models incorporate human-derived, physiologically relevant versions of all cell types that normally form part of the lungs anti-viral response. This review proposes a guideline using human induced pluripotent stem cells (iPSCs) to create all the disease-relevant cell types. iPSCs can be differentiated into lung epithelium, innate immune cells, endothelial cells, and fibroblasts at a large scale, recapitulating in vivo functions and providing genetic tractability. We advocate for building comprehensive iPSC-derived in vitro models of both proximal and distal lung regions to better understand and model respiratory infections, including interactions with chronic lung diseases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones del Sistema Respiratorio / Virosis / Células Madre Pluripotentes Inducidas / Pulmón Límite: Animals / Humans Idioma: En Revista: Respir Res Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones del Sistema Respiratorio / Virosis / Células Madre Pluripotentes Inducidas / Pulmón Límite: Animals / Humans Idioma: En Revista: Respir Res Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido