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Collective peroxide detoxification determines microbial mutation rate plasticity in E. coli.
Green, Rowan; Wang, Hejie; Botchey, Carol; Zhang, Siu Nam Nancy; Wadsworth, Charles; Tyrrell, Francesca; Letton, James; McBain, Andrew J; Paszek, Pawel; Krasovec, Rok; Knight, Christopher G.
Afiliación
  • Green R; School of Natural Sciences, Faculty of Science & Engineering, University of Manchester, United Kingdom.
  • Wang H; School of Biological Sciences, Faculty of Biology, Medicine & Health, University of Manchester, United Kingdom.
  • Botchey C; School of Biological Sciences, Faculty of Biology, Medicine & Health, University of Manchester, United Kingdom.
  • Zhang SNN; School of Biological Sciences, Faculty of Biology, Medicine & Health, University of Manchester, United Kingdom.
  • Wadsworth C; School of Biological Sciences, Faculty of Biology, Medicine & Health, University of Manchester, United Kingdom.
  • Tyrrell F; School of Biological Sciences, Faculty of Biology, Medicine & Health, University of Manchester, United Kingdom.
  • Letton J; School of Biological Sciences, Faculty of Biology, Medicine & Health, University of Manchester, United Kingdom.
  • McBain AJ; School of Health Sciences, Faculty of Biology Medicine & Health, University of Manchester, United Kingdom.
  • Paszek P; School of Biological Sciences, Faculty of Biology, Medicine & Health, University of Manchester, United Kingdom.
  • Krasovec R; Department of Biosystems and Soft Matter, Institute of Fundamental Technological Research, Polish Academy of Sciences, Warsaw, Poland.
  • Knight CG; School of Biological Sciences, Faculty of Biology, Medicine & Health, University of Manchester, United Kingdom.
PLoS Biol ; 22(7): e3002711, 2024 Jul.
Article en En | MEDLINE | ID: mdl-39008532
ABSTRACT
Mutagenesis is responsive to many environmental factors. Evolution therefore depends on the environment not only for selection but also in determining the variation available in a population. One such environmental dependency is the inverse relationship between mutation rates and population density in many microbial species. Here, we determine the mechanism responsible for this mutation rate plasticity. Using dynamical computational modelling and in culture mutation rate estimation, we show that the negative relationship between mutation rate and population density arises from the collective ability of microbial populations to control concentrations of hydrogen peroxide. We demonstrate a loss of this density-associated mutation rate plasticity (DAMP) when Escherichia coli populations are deficient in the degradation of hydrogen peroxide. We further show that the reduction in mutation rate in denser populations is restored in peroxide degradation-deficient cells by the presence of wild-type cells in a mixed population. Together, these model-guided experiments provide a mechanistic explanation for DAMP, applicable across all domains of life, and frames mutation rate as a dynamic trait shaped by microbial community composition.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Escherichia coli / Tasa de Mutación Idioma: En Revista: PLoS Biol Asunto de la revista: BIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Escherichia coli / Tasa de Mutación Idioma: En Revista: PLoS Biol Asunto de la revista: BIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos