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Increased levels of thymidine kinase 1 in malignant cell-derived extracellular vesicles.
Doulabi, Ehsan Manouchehri; Dubois, Louise; Löf, Liza; Sinha, Tanay Kumar; Harinck, George Mickhael; Stålhandske, Per; Larsson, Anders; Kamali-Moghaddam, Masood.
Afiliación
  • Doulabi EM; Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, SE-751 08, Uppsala, Sweden.
  • Dubois L; Department of Medical Sciences, Clinical Chemistry, Uppsala University, SE-751 85, Uppsala, Sweden.
  • Löf L; Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, SE-751 08, Uppsala, Sweden.
  • Sinha TK; Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, SE-751 08, Uppsala, Sweden.
  • Harinck GM; Department of Immunology, Genetics & Pathology, Science for Life Laboratory, Uppsala University, SE-751 08, Uppsala, Sweden.
  • Stålhandske P; Department of Medical Sciences, Clinical Chemistry, Uppsala University, SE-751 85, Uppsala, Sweden.
  • Larsson A; Biovica International AB, Dag Hammarskjölds väg 54B, Uppsala Science Park, SE-752 37, Uppsala, Sweden.
  • Kamali-Moghaddam M; Department of Medical Sciences, Clinical Chemistry, Uppsala University, SE-751 85, Uppsala, Sweden.
Biochem Biophys Rep ; 39: 101761, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39006942
ABSTRACT
Extracellular vesicles (EVs), whose main subtypes are exosomes, microparticles, and apoptotic bodies, are secreted by all cells and harbor biomolecules such as DNA, RNA, and proteins. They function as intercellular messengers and, depending on their cargo, may have multiple roles in cancer development. Thymidine kinase 1 (TK1) is a cell cycle-dependent enzyme used as a biomarker for cell proliferation. TK1 is usually elevated in cancer patients' serum, making the enzyme a valuable tumor proliferation biomarker that strongly correlates with cancer stage and metastatic capabilities. Here, we investigated the presence of TK1 in EVs derived from three prostate cancer cell lines with various p53 mutation statuses (LNCaP, PC3, and DU145), EVs from the normal prostate epithelial cell line RWPE-1 and EVs isolated from human seminal fluid (prostasomes). We measured the TK1 activity by a real-time assay for these EVs. We demonstrated that the TK1 enzyme activity is higher in EVs derived from the malignant cell lines, with the highest activity from cells deriving from the most aggressive cancer, compared to the prostasomes and RWPE-1 EVs. The measurement of TK1 activity in EVs may be essential in future prostate cancer studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biochem Biophys Rep Año: 2024 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biochem Biophys Rep Año: 2024 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Países Bajos