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The Porous SilMA Hydrogel Scaffolds Carrying Dual-Sensitive Paclitaxel Nanoparticles Promote Neuronal Differentiation for Spinal Cord Injury Repair.
Li, Zhixiang; Zhou, Tao; Bao, Zhengqi; Wu, Min; Mao, Yingji.
Afiliación
  • Li Z; School of Life Sciences, Bengbu Medical University, 2600 Donghai Road, Bengbu, 233030, China.
  • Zhou T; Department of Orthopedics, The First Affiliated Hospital of Bengbu Medical University, 287 Changhuai Road, Bengbu, 233004, China.
  • Bao Z; Department of Orthopedics, The First Affiliated Hospital of Bengbu Medical University, 287 Changhuai Road, Bengbu, 233004, China.
  • Wu M; Department of Orthopedics, The First Affiliated Hospital of Bengbu Medical University, 287 Changhuai Road, Bengbu, 233004, China.
  • Mao Y; Department of Orthopedics, The First Affiliated Hospital of Bengbu Medical University, 287 Changhuai Road, Bengbu, 233004, China. wumin200207@163.com.
Tissue Eng Regen Med ; 21(6): 809-827, 2024 Aug.
Article en En | MEDLINE | ID: mdl-39004636
ABSTRACT

BACKGROUND:

In the intricate pathological milieu post-spinal cord injury (SCI), neural stem cells (NSCs) frequently differentiate into astrocytes rather than neurons, significantly limiting nerve repair. Hence, the utilization of biocompatible hydrogel scaffolds in conjunction with exogenous factors to foster the differentiation of NSCs into neurons has the potential for SCI repair.

METHODS:

In this study, we engineered a 3D-printed porous SilMA hydrogel scaffold (SM) supplemented with pH-/temperature-responsive paclitaxel nanoparticles (PTX-NPs). We analyzed the biocompatibility of a specific concentration of PTX-NPs and its effect on NSC differentiation. We also established an SCI model to explore the ability of composite scaffolds for in vivo nerve repair.

RESULTS:

The physical adsorption of an optimal PTX-NPs dosage can simultaneously achieve pH/temperature-responsive release and commendable biocompatibility, primarily reflected in cell viability, morphology, and proliferation. An appropriate PTX-NPs concentration can steer NSC differentiation towards neurons over astrocytes, a phenomenon that is also efficacious in simulated injury settings. Immunoblotting analysis confirmed that PTX-NPs-induced NSC differentiation occurred via the MAPK/ERK signaling cascade. The repair of hemisected SCI in rats demonstrated that the composite scaffold augmented neuronal regeneration at the injury site, curtailed astrocyte and fibrotic scar production, and enhanced motor function recovery in rat hind limbs.

CONCLUSION:

The scaffold's porous architecture serves as a cellular and drug carrier, providing a favorable microenvironment for nerve regeneration. These findings corroborate that this strategy amplifies neuronal expression within the injury milieu, significantly aiding in SCI repair.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Traumatismos de la Médula Espinal / Diferenciación Celular / Paclitaxel / Ratas Sprague-Dawley / Hidrogeles / Nanopartículas / Andamios del Tejido / Células-Madre Neurales / Neuronas Límite: Animals Idioma: En Revista: Tissue Eng Regen Med Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación:
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Traumatismos de la Médula Espinal / Diferenciación Celular / Paclitaxel / Ratas Sprague-Dawley / Hidrogeles / Nanopartículas / Andamios del Tejido / Células-Madre Neurales / Neuronas Límite: Animals Idioma: En Revista: Tissue Eng Regen Med Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: