Your browser doesn't support javascript.
loading
Qualitative and quantitative determination of xylazine in oral fluid.
Levitas, Matthew; Thomas, Christopher; Widman, Corey; DeColumna, Joseph; Allgaier, Brandi; Conley, Eric; deHagen, Troy; Freitas, Isabela; Horvath, Hannah; Lemberg, Bridget; Lemberg, Dave.
Afiliación
  • Levitas M; Forensic Fluids Laboratories, 225 Parsons Street, Kalamazoo, MI 49007, USA.
  • Thomas C; Forensic Fluids Laboratories, 225 Parsons Street, Kalamazoo, MI 49007, USA.
  • Widman C; Forensic Fluids Laboratories, 225 Parsons Street, Kalamazoo, MI 49007, USA.
  • DeColumna J; Forensic Fluids Laboratories, 225 Parsons Street, Kalamazoo, MI 49007, USA.
  • Allgaier B; Forensic Fluids Laboratories, 225 Parsons Street, Kalamazoo, MI 49007, USA.
  • Conley E; Forensic Fluids Laboratories, 225 Parsons Street, Kalamazoo, MI 49007, USA.
  • deHagen T; Forensic Fluids Laboratories, 225 Parsons Street, Kalamazoo, MI 49007, USA.
  • Freitas I; Forensic Fluids Laboratories, 225 Parsons Street, Kalamazoo, MI 49007, USA.
  • Horvath H; Forensic Fluids Laboratories, 225 Parsons Street, Kalamazoo, MI 49007, USA.
  • Lemberg B; Forensic Fluids Laboratories, 225 Parsons Street, Kalamazoo, MI 49007, USA.
  • Lemberg D; Forensic Fluids Laboratories, 225 Parsons Street, Kalamazoo, MI 49007, USA.
J Anal Toxicol ; 48(7): 482-488, 2024 Aug 21.
Article en En | MEDLINE | ID: mdl-39002106
ABSTRACT
Xylazine has emerged in recent years as a dangerous adulterant in illicit fentanyl use, and methods for the detection of xylazine in toxicology panels are still lagging. We developed methods for the screening and quantitation of xylazine in oral fluid (OF), a popular testing medium due to its ease of collection and reflection of presence in blood for many classes of drugs. Enzyme-linked immunosorbent assays were employed for the rapid screening of xylazine directly from the collection device buffer with a cutoff of 1 ng/mL. Solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry facilitated the confirmation and quantification of xylazine as low as 0.1 ng/mL and a dynamic range of 0.1-25 ng/mL. Selectivity, ionization suppression, processed sample stability, and dilution effect were also assessed. The method was validated through the American National Standards Institute/American Academy of Forensic Sciences Standards Board (ANSI/ASB) Standard 036, first edition from 2019, and found to be accurate, precise, and robust. Living human subject OF samples collected within substance use disorder and therapeutic drug monitoring clinics received between September 2023 and January 2024, with the specific request to test for xylazine (n = 57), were screened. Presumptive positive samples were confirmed using the validated method. Xylazine confirmed living human subject OF sample concentrations ranged from 1.2 to 23.3 ng/mL.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saliva / Xilazina / Detección de Abuso de Sustancias / Espectrometría de Masas en Tándem / Extracción en Fase Sólida Límite: Humans Idioma: En Revista: J Anal Toxicol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saliva / Xilazina / Detección de Abuso de Sustancias / Espectrometría de Masas en Tándem / Extracción en Fase Sólida Límite: Humans Idioma: En Revista: J Anal Toxicol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido