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Visual Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid On-Site Detection of Escherichia coli O157: H7 in Milk Products.
Cui, Shuangshuang; Wei, Yong; Li, Can; Zhang, Jian; Zhao, Yunfeng; Peng, Xiayu; Sun, Fengxia.
Afiliación
  • Cui S; School of Food Science and Technology, Shihezi University, Shihezi 832000, China.
  • Wei Y; Key Laboratory for Food Nutrition and Safety Control of Xinjiang Production and Construction Corps, School of Food Science and Technology, Shihezi University, Shihezi 832000, China.
  • Li C; Xinjiang Tianrun Dairy Co., Ltd., Wuchang Road No. 2702, Urumqi 830000, China.
  • Zhang J; School of Food Science and Technology, Shihezi University, Shihezi 832000, China.
  • Zhao Y; Key Laboratory for Food Nutrition and Safety Control of Xinjiang Production and Construction Corps, School of Food Science and Technology, Shihezi University, Shihezi 832000, China.
  • Peng X; School of Food Science and Technology, Shihezi University, Shihezi 832000, China.
  • Sun F; Key Laboratory for Food Nutrition and Safety Control of Xinjiang Production and Construction Corps, School of Food Science and Technology, Shihezi University, Shihezi 832000, China.
Foods ; 13(13)2024 Jul 05.
Article en En | MEDLINE | ID: mdl-38998648
ABSTRACT
(1)

Background:

Rapid on-site testing is an effective method for the detection of Escherichia coli O157 H7(E. coli O157 H7) in food ingredients and the environment. (2)

Methods:

In this study, we developed colorimetric loop-mediated isothermal amplification (LAMP) and immunochromatographic test strips (ICTs) for the rapid and visual detection of E. coli O157 H7. This study designed new specific LAMP primers for E. coli O157 H7 virulence island genes. After the LAMP amplification, the double-stranded DNA target sequence labeled with digoxin and fluorescein isothiocyanate (FITC) at both ends was bound to the anti-digoxin antibody on the gold nanoparticles. Subsequently, it was further bound to the anti-FITC antibody at the T line of the ICTs, forming a positive test result. Hydroxynaphthyl blue dye was directly added to the LAMP amplification product. A blue color indicated positive results, while a purple color indicated negative results. (3)

Results:

Two visualization methods showed high specificity for the target strains. The visualization tests had sensitivities of 5.7 CFU mL-1, and the detection limit of the Escherichia coli O157 H7 in artificially contaminated milk samples was 5.7 × 102 CFU mL-1, which was consistent with the results of the standard method (LAMP-electrophoresis method) used in commercial inspection. (4)

Conclusions:

Both methods could be useful in remote and under-resourced areas.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Foods Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Foods Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza