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Hepatoprotective effects of resveratrol on α-amanitin-induced liver toxicity in rats.
Gezer, Arzu; Ustundag, Hilal; Mendil, Ali Sefa; Bedir, Gursel; Duysak, Lale.
Afiliación
  • Gezer A; Vocational School of Health Services, Atatürk University, Erzurum, Türkiye; Pharmaceutical Research and Development, Graduate School of Natural and Applied Sciences, Atatürk University, Erzurum, Türkiye. Electronic address: arzu.gezer@atauni.edu.tr.
  • Ustundag H; Erzincan Binali Yildirim University, Faculty of Medicine, Department of Physiology, Erzincan, Türkiye. Electronic address: hilal.ustundag@erzincan.edu.tr.
  • Mendil AS; Erciyes University, Faculty of Veterinary Medicine, Department of Pathology, Kayseri, Türkiye.
  • Bedir G; Atatürk University, School of Medicine, Department of Histology and Embryology, Erzurum, Türkiye.
  • Duysak L; Atatürk University, Faculty of Pharmacy, Department of Biochemistry, Erzurum, Türkiye.
Toxicon ; 247: 107855, 2024 Aug 28.
Article en En | MEDLINE | ID: mdl-38996975
ABSTRACT

OBJECTIVE:

The hepatoprotective effects of resveratrol against α-Amanitin (α-AMA)-induced liver toxicity were investigated in an experimental rat model, focusing on oxidative stress, inflammation, apoptosis, and liver function.

METHODS:

Thirty-two male Sprague-Dawley rats were divided into four groups (n = 8 per group) Control, resveratrol, α-AMA, and resveratrol+α-AMA. The resveratrol group received 20 mg/kg resveratrol orally for 7 days. The α-AMA group received 3 mg/kg α-AMA intraperitoneally on the 8th day. The resveratrol+α-AMA group received 20 mg/kg resveratrol orally (7 days) followed by 3 mg/kg α-AMA intraperitoneally on the 8th day. Liver tissues and blood samples were collected 48 h after α-amanitin administration for histopathological, immunohistochemical (NFkB, LC3B), and biochemical analyses (GSH, MDA, CAT, GPx, MPO, NOS, AST, ALT).

RESULTS:

α-AMA significantly increased AST and ALT levels, oxidative stress marker (MDA), and inflammatory marker (MPO), while reducing antioxidant levels (GSH, CAT, GPx) and NOS concentration (P < 0.001 for all parameters). Histopathological analysis showed severe liver damage with increased NFkB and LC3B expression. resveratrol treatment significantly reduced AST and ALT levels (P < 0.01 for both parameters), decreased MDA and MPO levels, and increased NOS concentration, GSH, CAT, and GPx levels (P < 0.05 for all parameters). Reduced NFkB and LC3B expression in the resveratrol+α-AMA group and showed histopathological improvements.

CONCLUSION:

Resveratrol demonstrated substantial hepatoprotective effects against α-AMA induced liver toxicity by reducing oxidative stress, inflammation, and apoptosis, and improving liver function. These findings suggest that resveratrol could be a potential therapeutic agent for treating liver damage caused by potent hepatotoxins like α-AMA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ratas Sprague-Dawley / Estrés Oxidativo / Alfa-Amanitina / Enfermedad Hepática Inducida por Sustancias y Drogas / Resveratrol / Hígado / Antioxidantes Límite: Animals Idioma: En Revista: Toxicon Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ratas Sprague-Dawley / Estrés Oxidativo / Alfa-Amanitina / Enfermedad Hepática Inducida por Sustancias y Drogas / Resveratrol / Hígado / Antioxidantes Límite: Animals Idioma: En Revista: Toxicon Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido