The recovery from taxane mediated apoptosis in PC-3 castration-resistant metastatic prostate cancer cells.

Guney Eskiler, Gamze; Deveci Ozkan, Asuman; Acikel Elmas, Merve; Ozturk, Melek; Arbak, Serap

Guney Eskiler, Gamze; Deveci Ozkan, Asuman; Acikel Elmas, Merve; Ozturk, Melek; Arbak, Serap.

Afiliación

Guney Eskiler G; Department of Medical Biology, Faculty of Medicine, Sakarya University, Sakarya, Turkey. Electronic address: gamzeguney@sakarya.edu.tr.
Deveci Ozkan A; Department of Medical Biology, Faculty of Medicine, Sakarya University, Sakarya, Turkey.
Acikel Elmas M; Department of Histology and Embryology, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey.
Ozturk M; Department of Medical Biology, Faculty of Medicine, Istanbul-Cerrahpasa University, Istanbul, Turkey.
Arbak S; Department of Histology and Embryology, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkey.

Toxicol In Vitro ; 100: 105894, 2024 Oct.

Article en En | MEDLINE | ID: mdl-38996827

ABSTRACT

ABSTRACT

Here, we revealed the reversibility of cabazitaxel (CBZ)-induced apoptosis in PC-3 castration resistant metastatic prostate cancer cells (mCRPC) through the hallmarks of apoptosis. The recovery of PC-3 cells from apoptosis upon removal of CBZ at different recovery periods was evaluated by Annexin V, DNA damage, oxidative damage, mitochondrial membrane depolarization, and caspase activation. Our results showed that the administration of CBZ caused apoptosis for 72 h in PC-3 cells. However, recovered cells exhibited decreased nuclear damage, plasma membrane disruption, ROS level, release cytochrome c level and caspase-3 activation with upregulation of Bcl-2 expression upon removal of especially 1 nM CBZ for 24 h recovery period in PC-3 cells. Our study indicates that CBZ treated PC-3 cells can recover after apoptotic cell death. However, advanced molecular analysis should elucidate the relationship between the molecular mechanisms of recovery and taxane response or resistance in PC-3 mCRPC cells.

Asunto(s)

Antineoplásicos; Apoptosis; Neoplasias de la Próstata Resistentes a la Castración; Especies Reactivas de Oxígeno; Taxoides; Masculino; Humanos; Apoptosis/efectos de los fármacos; Neoplasias de la Próstata Resistentes a la Castración/tratamiento farmacológico; Neoplasias de la Próstata Resistentes a la Castración/patología; Taxoides/farmacología; Antineoplásicos/farmacología; Especies Reactivas de Oxígeno/metabolismo; Proteínas Proto-Oncogénicas c-bcl-2/metabolismo; Proteínas Proto-Oncogénicas c-bcl-2/genética; Caspasa 3/metabolismo; Células PC-3; Citocromos c/metabolismo; Potencial de la Membrana Mitocondrial/efectos de los fármacos; Daño del ADN/efectos de los fármacos; Línea Celular Tumoral

Palabras clave

Apoptosis; Cabazitaxel; Castration resistant metastatic prostate cancer cells; Drug resistance; Taxanes

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Especies Reactivas de Oxígeno / Apoptosis / Taxoides / Neoplasias de la Próstata Resistentes a la Castración / Antineoplásicos Límite: Humans / Male Idioma: En Revista: Toxicol In Vitro Asunto de la revista: TOXICOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido

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