A type III-Dv CRISPR-Cas system is controlled by the transcription factor RpaB and interacts with the DEAD-box RNA helicase CrhR.

Bilger, Raphael; Migur, Angela; Wulf, Alexander; Steglich, Claudia; Urlaub, Henning; Hess, Wolfgang R

Bilger, Raphael; Migur, Angela; Wulf, Alexander; Steglich, Claudia; Urlaub, Henning; Hess, Wolfgang R.

Afiliación

Bilger R; Faculty of Biology, Genetics and Experimental Bioinformatics, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany.
Migur A; Faculty of Biology, Genetics and Experimental Bioinformatics, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany.
Wulf A; Bioanalytics Research Group, Department of Clinical Chemistry, University Medical Centre, 37075 Göttingen, Germany; Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany.
Steglich C; Faculty of Biology, Genetics and Experimental Bioinformatics, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany.
Urlaub H; Bioanalytics Research Group, Department of Clinical Chemistry, University Medical Centre, 37075 Göttingen, Germany; Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany.
Hess WR; Faculty of Biology, Genetics and Experimental Bioinformatics, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany. Electronic address: wolfgang.hess@biologie.uni-freiburg.de.

Cell Rep ; 43(7): 114485, 2024 Jul 23.

Article en En | MEDLINE | ID: mdl-38996066

ABSTRACT

ABSTRACT

How CRISPR-Cas systems defend bacteria and archaea against invading genetic elements is well understood, but less is known about their regulation. In the cyanobacterium Synechocystis sp. PCC 6803, the expression of one of the three different CRISPR-Cas systems responds to changes in environmental conditions. The cas operon promoter of this system is controlled by the light- and redox-responsive transcription factor RpaB binding to an HLR1 motif, resulting in transcriptional activation at low light intensities. However, the strong promoter that drives transcription of the cognate repeat-spacer array is not controlled by RpaB. Instead, the leader transcript is bound by the redox-sensitive RNA helicase CrhR. Crosslinking coupled with mass spectrometry analysis and site-directed mutagenesis revealed six residues involved in the CrhR-RNA interaction, with C371 being critically important. Thus, the expression of a type III-Dv CRISPR-Cas system is linked to the redox status of the photosynthetic cell at the transcriptional and post-transcriptional levels.

Asunto(s)

Proteínas Bacterianas; Sistemas CRISPR-Cas; ARN Helicasas DEAD-box; Synechocystis; Sistemas CRISPR-Cas/genética; ARN Helicasas DEAD-box/metabolismo; ARN Helicasas DEAD-box/genética; Proteínas Bacterianas/metabolismo; Proteínas Bacterianas/genética; Synechocystis/metabolismo; Synechocystis/genética; Factores de Transcripción/metabolismo; Factores de Transcripción/genética; Regiones Promotoras Genéticas/genética; Unión Proteica

Palabras clave

CP: Microbiology; CP: Molecular biology; CRISPR leader; CRISPR-Cas systems; CrhR DEAD-box RNA helicase; RNA structure; cyanobacteria; regulation of CRISPR-Cas gene expression; transcription factor RpaB

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Synechocystis / ARN Helicasas DEAD-box / Sistemas CRISPR-Cas Idioma: En Revista: Cell Rep Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos

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