3D Bioprinting with Visible Light Cross-Linkable Mucin-Hyaluronic Acid Composite Bioink for Lung Tissue Engineering.

Sasikumar, Sruthi C; Goswami, Upashi; Raichur, Ashok M

Sasikumar, Sruthi C; Goswami, Upashi; Raichur, Ashok M.

Afiliación

Sasikumar SC; Department of Materials Engineering, Indian Institute of Science Bangalore, Karnataka 560012, India.
Goswami U; Department of Materials Engineering, Indian Institute of Science Bangalore, Karnataka 560012, India.
Raichur AM; Department of Materials Engineering, Indian Institute of Science Bangalore, Karnataka 560012, India.

ACS Appl Bio Mater ; 7(8): 5411-5422, 2024 Aug 19.

Article en En | MEDLINE | ID: mdl-38996006

ABSTRACT

ABSTRACT

3D printing can revolutionize personalized medicine by allowing cost-effective, customized tissue-engineering constructs. However, the limited availability and diversity of biopolymeric hydrogels restrict the variety and applications of bioinks. In this study, we introduce a composite bioink for 3D bioprinting, combining a photo-cross-linkable derivative of Mucin (Mu) called Methacrylated Mucin (MuMA) and Hyaluronic acid (HA). The less explored Mucin is responsible for the hydrogel nature of mucus and holds the potential to be used as a bioink material because of its plethora of features. HA, a crucial extracellular matrix component, is mucoadhesive and enhances ink viscosity and printability. Photo-cross-linking with 405 nm light stabilizes the printed scaffolds without damaging cells. Rheological tests reveal shear-thinning behavior, aiding cell protection during printing and improved MuMA bioink viscosity by adding HA. The printed structures exhibited porous behavior conducive to nutrient transport and cell migration. After 4 weeks in phosphate-buffered saline, the scaffolds retain 70% of their mass, highlighting stability. Biocompatibility tests with lung epithelial cells (L-132) confirm cell attachment and growth, suggesting suitability for lung tissue engineering. It is envisioned that the versatility of bioink could lead to significant advancements in lung tissue engineering and various other biomedical applications.

Asunto(s)

Materiales Biocompatibles; Bioimpresión; Ácido Hialurónico; Ensayo de Materiales; Mucinas; Impresión Tridimensional; Ingeniería de Tejidos; Ácido Hialurónico/química; Ácido Hialurónico/farmacología; Materiales Biocompatibles/química; Materiales Biocompatibles/farmacología; Humanos; Mucinas/química; Mucinas/metabolismo; Tinta; Luz; Pulmón/citología; Tamaño de la Partícula; Andamios del Tejido/química; Hidrogeles/química; Hidrogeles/farmacología

Palabras clave

3D bioprinting; bioink; hyaluronic acid; lung tissue engineering; mucin; scaffolds

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Materiales Biocompatibles / Ensayo de Materiales / Ingeniería de Tejidos / Bioimpresión / Impresión Tridimensional / Ácido Hialurónico / Mucinas Límite: Humans Idioma: En Revista: ACS Appl Bio Mater Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google