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Oxygen carriers affect kidney immunogenicity during ex-vivo machine perfusion.
Rother, Tamina; Horgby, Carina; Schmalkuche, Katharina; Burgmann, Jonathan M; Nocke, Fabian; Jägers, Johannes; Schmitz, Jessica; Bräsen, Jan Hinrich; Cantore, Miriam; Zal, Franck; Ferenz, Katja B; Blasczyk, Rainer; Figueiredo, Constanca.
Afiliación
  • Rother T; Institute of Transfusion Medicine and Transplant Engineering, Hannover Medical School, Hannover, Germany.
  • Horgby C; Institute of Transfusion Medicine and Transplant Engineering, Hannover Medical School, Hannover, Germany.
  • Schmalkuche K; Institute of Transfusion Medicine and Transplant Engineering, Hannover Medical School, Hannover, Germany.
  • Burgmann JM; Institute of Transfusion Medicine and Transplant Engineering, Hannover Medical School, Hannover, Germany.
  • Nocke F; Institute of Physiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
  • Jägers J; Department of Biomedicine, Aarhus University, Aarhus, Denmark.
  • Schmitz J; Nephropathology Unit, Institute of Pathology, Hannover Medical School, Hannover, Germany.
  • Bräsen JH; Nephropathology Unit, Institute of Pathology, Hannover Medical School, Hannover, Germany.
  • Cantore M; Institute of Physiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
  • Zal F; Hemarina SA, Aéropôle Centre, Morlaix, France.
  • Ferenz KB; Institute of Physiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
  • Blasczyk R; CeNIDE (Center for Nanointegration Duisburg-Essen), University of Duisburg-Essen, Duisburg, Germany.
  • Figueiredo C; Institute of Transfusion Medicine and Transplant Engineering, Hannover Medical School, Hannover, Germany.
Front Transplant ; 2: 1183908, 2023.
Article en En | MEDLINE | ID: mdl-38993849
ABSTRACT
Normothermic ex-vivo machine perfusion provides a powerful tool to improve donor kidney preservation and a route for the delivery of pharmacological or gene therapeutic interventions prior to transplantation. However, perfusion at normothermic temperatures requires adequate tissue oxygenation to meet the physiological metabolic demand. For this purpose, the addition of appropriate oxygen carriers (OCs) to the perfusion solution is essential to ensure a sufficient oxygen supply and reduce the risk for tissue injury due to hypoxia. It is crucial that the selected OCs preserve the integrity and low immunogenicity of the graft. In this study, the effect of two OCs on the organ's integrity and immunogenicity was evaluated. Porcine kidneys were perfused ex-vivo for four hours using perfusion solutions supplemented with red blood cells (RBCs) as conventional OC, perfluorocarbon (PFC)-based OC, or Hemarina-M101 (M101), a lugworm hemoglobin-based OC named HEMO2life®, recently approved in Europe (i.e., CE obtained in October 2022). Perfusions with all OCs led to decreased lactate levels. Additionally, none of the OCs negatively affected renal morphology as determined by histological analyses. Remarkably, all OCs improved the perfusion solution by reducing the expression of pro-inflammatory mediators (IL-6, IL-8, TNFα) and adhesion molecules (ICAM-1) on both transcript and protein level, suggesting a beneficial effect of the OCs in maintaining the low immunogenicity of the graft. Thus, PFC-based OCs and M101 may constitute a promising alternative to RBCs during normothermic ex-vivo kidney perfusion.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Transplant Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Transplant Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza