Establishing Three-Dimensional Explant Culture of Human Dental Pulp Tissue.

Seo, Eun Jin; Park, Soyoung; Lee, Eungyung; Huh, Yang Hoon; Ha, Ye Eun; Tigyi, Gabor J; Jeong, Taesung; Jang, Il Ho; Shin, Jonghyun

Seo, Eun Jin; Park, Soyoung; Lee, Eungyung; Huh, Yang Hoon; Ha, Ye Eun; Tigyi, Gabor J; Jeong, Taesung; Jang, Il Ho; Shin, Jonghyun.

Afiliación

Seo EJ; Dental and Life Science Institute, School of Dentisty, Pusan National University, Yangsan, Korea.
Park S; Tissue Regeneration Laboratory, StemDen Co., Ltd., Yangsan, Korea.
Lee E; Dental and Life Science Institute, School of Dentisty, Pusan National University, Yangsan, Korea.
Huh YH; Department of Pediatric Dentistry, School of Dentistry, Pusan National University, Yangsan, Korea.
Ha YE; Dental Research Institute, Pusan National University Dental Hospital, Yangsan, Korea.
Tigyi GJ; Dental and Life Science Institute, School of Dentisty, Pusan National University, Yangsan, Korea.
Jeong T; Department of Pediatric Dentistry, School of Dentistry, Pusan National University, Yangsan, Korea.
Jang IH; Dental Research Institute, Pusan National University Dental Hospital, Yangsan, Korea.
Shin J; Center for Electron Microscopy Research, Korea Basic Science Institute, Ochang, Korea.

Int J Stem Cells ; 17(3): 330-336, 2024 Aug 30.

Article en En | MEDLINE | ID: mdl-38993099

ABSTRACT

ABSTRACT

Mesenchymal stem cells in the dental tissue indicate a disposition for differentiation into diverse dental lineages and contain enormous potential as the important means for regenerative medicine in dentistry. Among various dental tissues, the dental pulp contains stem cells, progenitor cells and odontoblasts for maintaining dentin homeostasis. The conventional culture of stem cells holds a limit as the living tissue constitutes the three-dimensional (3D) structure. Recent development in the organoid cultures have successfully recapitulated 3D structure and advanced to the assembling of different types. In the current study, the protocol for 3D explant culture of the human dental pulp tissue has been established by adopting the organoid culture. After isolating dental pulp from human tooth, the intact tissue was placed between two layers for Matrigel with addition of the culture medium. The reticular outgrowth of pre-odontoblast layer continued for a month and the random accumulation of dentin was observed near the end. Electron microscopy showed the cellular organization and in situ development of dentin, and immunohistochemistry exhibited the expression of odontoblast and stem cell markers in the outgrowth area. Three-dimensional explant culture of human dental pulp will provide a novel platform for understanding stem cell biology inside the tooth and developing the regenerative medicine.

Palabras clave

Cell culture techniques; Dental pulp stem cells; Explant culture; Odontoblasts; three dimensional

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Int J Stem Cells Año: 2024 Tipo del documento: Article Pais de publicación: