Your browser doesn't support javascript.
loading
Exploring cell death mechanisms in spheroid cultures using a novel application of the RIP3-caspase3-assay.
Philippi, C I; Hagens, J; Heuer, K M; Schmidt, H C; Schuppert, P; Pagerols Raluy, L; Trochimiuk, M; Li, Z; Bunders, M J; Reinshagen, K; Tomuschat, C.
Afiliación
  • Philippi CI; Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
  • Hagens J; Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
  • Heuer KM; Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
  • Schmidt HC; Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
  • Schuppert P; Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
  • Pagerols Raluy L; Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
  • Trochimiuk M; Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
  • Li Z; Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
  • Bunders MJ; Research Department of Virus Immunology, Leibniz Institute of Virology, Hamburg, Germany.
  • Reinshagen K; Division of Regenerative Medicine and Immunology, III. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
  • Tomuschat C; Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Sci Rep ; 14(1): 16032, 2024 07 11.
Article en En | MEDLINE | ID: mdl-38992075
ABSTRACT
This study explores the application of the RIP3-caspase3-assay in heterogeneous spheroid cultures to analyze cell death pathways, emphasizing the nuanced roles of apoptosis and necroptosis. By employing directly conjugated monoclonal antibodies, we provide detailed insights into the complex mechanisms of cell death. Our findings demonstrate the assay's capability to differentiate between RIP1-independent apoptosis, necroptosis, and RIP1-dependent apoptosis, marking a significant advancement in organoid research. Additionally, we investigate the effects of TNFα on isolated intestinal epithelial cells, revealing a concentration-dependent response and an adaptive or threshold reaction to TNFα-induced stress. The results indicate a preference for RIP1-independent cell death pathways upon TNFα stimulation, with a notable increase in apoptosis and a secondary role of necroptosis. Our research underscores the importance of the RIP3-caspase3-assay in understanding cell death mechanisms in organoid cultures, offering valuable insights for disease modeling and the development of targeted therapies. The assay's adaptability and robustness in spheroid cultures enhances its potential as a tool in personalized medicine and translational research.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Apoptosis / Esferoides Celulares / Caspasa 3 / Proteína Serina-Treonina Quinasas de Interacción con Receptores / Necroptosis Límite: Humans Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Apoptosis / Esferoides Celulares / Caspasa 3 / Proteína Serina-Treonina Quinasas de Interacción con Receptores / Necroptosis Límite: Humans Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido