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Iron protoporphyrin IX-hyaluronan hydrogel-supported luminol chemiluminescence for the detection of nitric oxide in physiological solutions.
Alsharabasy, Amir M; Sankar, Magesh; Biggs, Manus; Farràs, Pau; Pandit, Abhay.
Afiliación
  • Alsharabasy AM; CÚRAM, SFI Research Centre for Medical Devices, University of Galway, H91 W2TY, Ireland. Electronic address: Amir.Abdo@universityofgalway.ie.
  • Sankar M; CÚRAM, SFI Research Centre for Medical Devices, University of Galway, H91 W2TY, Ireland.
  • Biggs M; CÚRAM, SFI Research Centre for Medical Devices, University of Galway, H91 W2TY, Ireland.
  • Farràs P; CÚRAM, SFI Research Centre for Medical Devices, University of Galway, H91 W2TY, Ireland; School of Biological and Chemical Sciences, Ryan Institute, University of Galway, H91 TK33, Ireland.
  • Pandit A; CÚRAM, SFI Research Centre for Medical Devices, University of Galway, H91 W2TY, Ireland. Electronic address: Abhay.pandit@universityofgalway.ie.
Talanta ; 278: 126522, 2024 Oct 01.
Article en En | MEDLINE | ID: mdl-38991408
ABSTRACT
Due to its role as a free radical signal-transducing agent with a short lifespan, precise measurement of nitric oxide (●NO) levels presents significant challenges. Various analytical techniques offer distinct advantages and disadvantages for ●NO detection. This research aims to simplify the detection process by developing a hydrogel system using iron(III)-protoporphyrin IX (hemin)-loaded hyaluronan for the detection of ●NO in solution. Various hydrogel formulations were created, and the effects of their components on hydrogel-supported luminol chemiluminescence (CL) kinetics, radical scavenging, and physicochemical properties were analysed through factorial analysis. The candidate formulations were then evaluated using two ●NO donors. An increase in the degree of crosslinking in unloaded formulations enhanced interactions with the CL reaction components, hydrogen peroxide (H2O2) and luminol, thereby affecting light generation. However, hemin loading negated these effects, resulting in more prominent luminescence kinetics in formulations with lower crosslinking degrees. Similarly, ●NO influenced the kinetics differently, interacting with both the CL reaction and hydrogel components. Hemin-loaded formulations exhibited enhanced signal propagation when exposed to ●NO, followed by H2O2 and luminol, whereas reversing the order of addition inhibited this propagation. The magnitude of these luminescence changes depended on the type and concentration of the ●NO donor, demonstrating greater sensitivity to ●NO levels compared to amperometric sensing. These findings suggest that the studied hydrogel platform has potential for the facile and accurate detection of ●NO in solution, requiring minimal sample sizes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Protoporfirinas / Hidrogeles / Ácido Hialurónico / Mediciones Luminiscentes / Luminol / Óxido Nítrico Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Protoporfirinas / Hidrogeles / Ácido Hialurónico / Mediciones Luminiscentes / Luminol / Óxido Nítrico Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos