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A prospective study of the proteome of equine pre-implantation embryo.
Teles Filho, Antônio Carlos de A; Sanchez, Deisy J D; Viana, Arabela G A; Sheheryar, Sheheryar; Guerreiro, Denise D; Bustamante-Filho, Ivan C; Martins, Aline M A; Sousa, Marcelo V; Ricart, Carlos A O; Fontes, Wagner; Moura, Arlindo A.
Afiliación
  • Teles Filho ACA; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.
  • Sanchez DJD; Laboratório de Biotecnologia, Universidad Cooperativa de Colombia, Bucaramanga, Colombia.
  • Viana AGA; Department of Biology, Federal University of Viçosa, Viçosa, Brazil.
  • Sheheryar S; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.
  • Guerreiro DD; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.
  • Bustamante-Filho IC; Laboratório de Biotecnologia, Universidade Do Vale Do Taquari, Lajeado, Brazil.
  • Martins AMA; Laboratory of Protein Chemistry and Biochemistry, University of Brasília, Brasilia, Brazil.
  • Sousa MV; Laboratory of Protein Chemistry and Biochemistry, University of Brasília, Brasilia, Brazil.
  • Ricart CAO; Laboratory of Protein Chemistry and Biochemistry, University of Brasília, Brasilia, Brazil.
  • Fontes W; Laboratory of Protein Chemistry and Biochemistry, University of Brasília, Brasilia, Brazil.
  • Moura AA; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.
Reprod Domest Anim ; 59(7): e14663, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38990011
ABSTRACT
The present study was conducted to investigate the global proteome of 8-day-old equine blastocysts. Follicular dynamics of eight adult mares were monitored by ultrasonography and inseminated 24 h after the detection of a preovulatory follicle. Four expanded blastocysts were recovered, pooled, and subjected to protein extraction and mass spectrometry. Protein identification was conducted based on four database searches (PEAKS, Proteome Discoverer software, SearchGUI software, and PepExplorer). Enrichment analysis was performed using gProfiler, Panther, and String platforms. After the elimination of identification redundancies among search tools (at three levels, based on identifiers, peptides, and cross-database mapping), 1977 proteins were reliably identified in the samples of equine embryos. Proteomic analysis unveiled robust metabolic activity in the 8-day equine embryo, highlighted by an abundance of proteins engaged in key metabolic pathways like the TCA cycle, ATP biosynthesis, and glycolysis. The prevalence of chaperones among highly abundant proteins suggests that regulation of protein folding, and degradation is a key process during embryo development. These findings pave the way for developing new strategies to improve equine embryo media and optimize in vitro fertilization techniques.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Blastocisto / Proteoma Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Blastocisto / Proteoma Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania