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Using SWATH-MS to identify new molecular biomarkers in gingival crevicular fluid for detecting periodontitis and its response to treatment.
Blanco-Pintos, T; Regueira-Iglesias, A; Relvas, M; Alonso-Sampedro, M; Chantada-Vázquez, M P; Balsa-Castro, C; Tomás, I.
Afiliación
  • Blanco-Pintos T; Oral Sciences Research Group, Special Needs Unit, Department of Surgery and Medical-Surgical Specialties, School of Medicine and Dentistry, Universidade de Santiago de Compostela, Health Research Institute of Santiago (IDIS), Santiago de Compostela, Spain.
  • Regueira-Iglesias A; Oral Sciences Research Group, Special Needs Unit, Department of Surgery and Medical-Surgical Specialties, School of Medicine and Dentistry, Universidade de Santiago de Compostela, Health Research Institute of Santiago (IDIS), Santiago de Compostela, Spain.
  • Relvas M; Oral Pathology and Rehabilitation Research Unit (UNIPRO), University Institute of Health Sciences (IUCS-CESPU), Gandra, Portugal.
  • Alonso-Sampedro M; Department of Internal Medicine and Clinical Epidemiology, Complejo Hospitalario Universitario, Health Research Institute of Santiago (IDIS), Santiago de Compostela, Spain.
  • Chantada-Vázquez MP; Proteomic Unit, Health Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
  • Balsa-Castro C; Oral Sciences Research Group, Special Needs Unit, Department of Surgery and Medical-Surgical Specialties, School of Medicine and Dentistry, Universidade de Santiago de Compostela, Health Research Institute of Santiago (IDIS), Santiago de Compostela, Spain.
  • Tomás I; Oral Sciences Research Group, Special Needs Unit, Department of Surgery and Medical-Surgical Specialties, School of Medicine and Dentistry, Universidade de Santiago de Compostela, Health Research Institute of Santiago (IDIS), Santiago de Compostela, Spain.
J Clin Periodontol ; 51(10): 1342-1358, 2024 Oct.
Article en En | MEDLINE | ID: mdl-38987231
ABSTRACT

AIM:

To identify new biomarkers to detect untreated and treated periodontitis in gingival crevicular fluid (GCF) using sequential window acquisition of all theoretical mass spectra (SWATH-MS). MATERIALS AND

METHODS:

GCF samples were collected from 44 periodontally healthy subjects and 40 with periodontitis (Stages III-IV). In the latter, 25 improved clinically 2 months after treatment. Samples were analysed using SWATH-MS, and proteins were identified by the UniProt human-specific database. The diagnostic capability of the proteins was determined with generalized additive models to distinguish the three clinical conditions.

RESULTS:

In the untreated periodontitis vs. periodontal health modelling, five proteins showed excellent or good bias-corrected (bc)-sensitivity/bc-specificity values of >80%. These were GAPDH, ZG16B, carbonic anhydrase 1, plasma protease inhibitor C1 and haemoglobin subunit beta. GAPDH with MMP-9, MMP-8, zinc-α-2-glycoprotein and neutrophil gelatinase-associated lipocalin and ZG16B with cornulin provided increased bc-sensitivity/bc-specificity of >95%. For distinguishing treated periodontitis vs. periodontal health, most of these proteins and their combinations revealed a predictive ability similar to previous modelling. No model obtained relevant results to differentiate between periodontitis conditions.

CONCLUSIONS:

New single and dual GCF protein biomarkers showed outstanding results in discriminating untreated and treated periodontitis from periodontal health. Periodontitis conditions were indistinguishable. Future research must validate these findings.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periodontitis / Biomarcadores / Líquido del Surco Gingival / Metaloproteinasa 9 de la Matriz / Metaloproteinasa 8 de la Matriz Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: J Clin Periodontol Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periodontitis / Biomarcadores / Líquido del Surco Gingival / Metaloproteinasa 9 de la Matriz / Metaloproteinasa 8 de la Matriz Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: J Clin Periodontol Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos