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Inhibition of dengue virus infection in vitro by fucoidan and polysaccharide extract from marine alga Sargassum spp.
Panwong, Suthida; Phinyo, Kittiya; Duangjan, Kritsana; Sattayawat, Pachara; Pekkoh, Jeeraporn; Tragoolpua, Yingmanee; Yenchitsomanus, Pa-Thai; Panya, Aussara.
Afiliación
  • Panwong S; Doctor of Philosophy Program in Applied Microbiology (International Program), Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Phinyo K; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center of Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, C
  • Duangjan K; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center of Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, C
  • Sattayawat P; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Cell Engineering for Cancer Therapy Research Group, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Pekkoh J; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Science and Technology Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center of Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, C
  • Tragoolpua Y; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Natural Extracts and Innovative Products for Alternative Healthcare Research Group, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Yenchitsomanus PT; Division of Molecular Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand; Siriraj Center of Research Excellence for Cancer Immunotherapy (SiCORE-CIT), Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
  • Panya A; Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Natural Extracts and Innovative Products for Alternative Healthcare Research Group, Chiang Mai University, Chiang Mai 50200, Thailand; Cell Engineering for Cancer Therapy Research Group, Faculty of Science,
Int J Biol Macromol ; 276(Pt 1): 133496, 2024 Sep.
Article en En | MEDLINE | ID: mdl-38986999
ABSTRACT
Dengue virus (DENV) infection poses a global health threat, leading to severe conditions with the potential for critical outcomes. Currently, there are no specific drugs available whereas the vaccine does not offer comprehensive protection across all DENV serotypes. Therefore, the development of potential antiviral agents is necessary to reduce the severity risk and interrupt the transmission circuit. The search for effective antiviral agents against DENV has predominantly focused on natural resources, particularly those demonstrating diverse biological activities and high safety profiles. Cyanobacteria and algae including Leptolyngbya sp., Spirulina sp., Chlorella sp., and Sargassum spp., which are prevalent species in Thailand, have been reported for their diverse biological activities and high safety profiles. However, their anti-DENV activity has not been documented. In this study, the screening assay was performed to compare the antiviral activity against DENV of crude polysaccharide and ethanolic extracts derived from 4 species of cyanobacteria and algae in Vero cells. Polysaccharide extracts from Sargassum spp. were the most effective in inhibiting DENV-2 infection under co-infection conditions, where the virus was exposed to the extract at the time of infection. Treatment of the extract significantly reduced the ability of DENV to bind to the host cells to 47.87 ± 3.88 % while treatment upon virus binding step had no antiviral effect suggesting the underlaying mechanism of the extract on interfering virus binding step. Fucoidan, a key bioactive substance in Sargassum polysaccharide, showed to reduce DENV-2 infection to 26.59 ± 5.01 %, 20.46 ± 6.58 % under the co-infection condition in Vero and A549 cells, respectively. In accompanied with Sargassum polysaccharide, fucoidan disturbed the virus binding to the host cells. These findings warrant further development and exploration of the Sargassum-derived polysaccharide, fucoidan, as a promising candidate for combating DENV infections.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antivirales / Polisacáridos / Dengue / Virus del Dengue / Sargassum Límite: Animals / Humans Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antivirales / Polisacáridos / Dengue / Virus del Dengue / Sargassum Límite: Animals / Humans Idioma: En Revista: Int J Biol Macromol Año: 2024 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Países Bajos