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The contribution of different sperm parameters to better explain ram semen cryoresistance.
Yánez-Ortiz, I; García, W; Tabarez, A; Palomo, M J.
Afiliación
  • Yánez-Ortiz I; Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, Bellaterra E-08193, Spain; School of Veterinary Medicine, Faculty of Medical, Health and Life Sciences, International University of Ecuador, Quito 170411, Ecuador.
  • García W; Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, Bellaterra E-08193, Spain.
  • Tabarez A; Faculty of Biological and Agricultural Sciences. University Veracruzana, Tuxpan, Veracruz MX-92876, Mexico.
  • Palomo MJ; Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, Bellaterra E-08193, Spain. Electronic address: mariajesus.palomo@uab.cat.
Vet J ; 306: 106194, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38986926
ABSTRACT
In order to determine an effective procedure for explaining ram sperm cryoresistance and develop a new model for breeders classification, a retrospective study was conducted using sperm analysis data obtained over two consecutive years from a total of 82 sessions of ram semen cryopreservation. In each session, fresh ejaculates from eight males were collected via artificial vagina, pooled and frozen in liquid nitrogen vapors. After thawing, a total of 19,084 sperm tracks and 11,319 morphometric measurements were analysed. Clustering analyses were applied to establish motile and morphometric sperm subpopulations. Additionally, plasma and acrosome membrane integrity, as well mitochondrial activity using flow cytometry immediately after sperm thawing and following hypoosmotic shock test (HOST) was assessed. To develop a Ram Sperm Cryoresistance Index, Principal Component Analyses (PCA) using 22 variables were conducted. In the first PCA, the parameters that best explain cryoresistance include total motility (TM), motile subpopulation 2 (motSP2, which groups slow, very linear spermatozoa with low lateral head displacement), morphometric subpopulation 1 (morphSP1, grouping spermatozoa with the smallest head size and lowest shape values), sperm plasma membrane integrity immediately after thawing and following hypoosmotic shock test. These parameters collectively account for 77.34 % of the accumulated variance. To emphasize their importance, a second PCA was performed, revealing significant higher weighting coefficients for the quantity (TM) and quality (motSP2) of sperm movement after thawing, compared to the head size and shape of the thawed sperm (morphSP1). Furthermore, HOST Viability played a more decisive role than what was observed under isotonic conditions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Semen / Espermatozoides / Criopreservación / Análisis de Semen Límite: Animals Idioma: En Revista: Vet J Asunto de la revista: MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación de Semen / Espermatozoides / Criopreservación / Análisis de Semen Límite: Animals Idioma: En Revista: Vet J Asunto de la revista: MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido