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Revealing critical functional enzymes in anammox nitrogen removal and rate-limiting step in catalytic pathways: Insight into metaproteomics and density functional theory.
Jiang, Zhicheng; He, Yuhang; Zeng, Ming; Zhang, Yinqing; Xu, Xinxin; Zhang, Meng.
Afiliación
  • Jiang Z; College of Marine and Environmental Sciences, Tianjin University of Science & Technology, Tianjin, China.
  • He Y; College of Marine and Environmental Sciences, Tianjin University of Science & Technology, Tianjin, China.
  • Zeng M; College of Marine and Environmental Sciences, Tianjin University of Science & Technology, Tianjin, China. Electronic address: ming.zeng@tust.edu.cn.
  • Zhang Y; Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China. Electronic address: yinqing.zhang@nankai.ed
  • Xu X; Department of Environmental Engineering, College of Environmental & Resource Sciences, Zhejiang University, Hangzhou, China.
  • Zhang M; Department of Environmental Engineering, College of Environmental & Resource Sciences, Zhejiang University, Hangzhou, China.
Bioresour Technol ; 406: 131090, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38986880
ABSTRACT
To reveal the key enzymes in the nitrogen removal pathway and to further elucidate the mechanism of the catalytic reaction, this study utilized metaproteomics combined with molecular dynamics and density functional theory calculation. K. stuttgartiensis provided the proteins up to 88.37 % in the anammox-based system. Hydrazine synthase (HZS) and hydrazine dehydrogenase (HDH) accounted for 15.94 % and 3.45 % of the total proteins expressed by K. stuttgartiensis, thus were considered as critical enzymes in the nitrogen removal pathway. The process of HZSγ binding to NO with lowest binding free energy of -4.91 ± 1.33 kJ/mol. The reaction catalyzed by HZSα was calculated to be the rate-limiting catalyzing step, because it transferred the proton from NH3 to ·OH by crossing an energy barrier of up to 190.29 kJ/mol. This study provided molecular level insights to enhance the performance of nitrogen removal in anammox-based system.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteómica / Nitrógeno Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteómica / Nitrógeno Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido