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PBMC-mediated modulation of macrophage polarization in RAW264.7 cells through STAT1/STAT6 signaling cascades.
Zhang, Wen-Bo; Chen, Zu-Xiang; Liu, Zhen; Qian, Xin-Yu; Ge, Yan-Zhi; Zhang, Hai-Yan; Xu, Wen-Ting; Shan, Le-Tian; Zhao, Dong-Bao.
Afiliación
  • Zhang WB; Department of Rheumatology and Immunology, Changhai Hospital, Naval Medical University, Shanghai 200433, China.
  • Chen ZX; The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China.
  • Liu Z; Department of Anatomy, Naval Medical University, Shanghai 200433, China.
  • Qian XY; Department of Rheumatology and Immunology, Changhai Hospital, Naval Medical University, Shanghai 200433, China.
  • Ge YZ; The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China.
  • Zhang HY; The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China.
  • Xu WT; The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China.
  • Shan LT; The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China. Electronic address: letian.shan@zcmu.edu.cn.
  • Zhao DB; Department of Rheumatology and Immunology, Changhai Hospital, Naval Medical University, Shanghai 200433, China. Electronic address: dongbaozhao@163.com.
Int Immunopharmacol ; 138: 112651, 2024 Sep 10.
Article en En | MEDLINE | ID: mdl-38986303
ABSTRACT
Peripheral blood mononuclear cells (PBMC), sourced autologously, offer numerous advantages when procured easier acquisition process, no in vitro amplification needed, decreased intervention and overall increased acceptability make PBMC an attractive candidate for cell therapy treatment. However, the exact mechanism by which PBMC treat diseases remains poorly understood. Immune imbalance is the pathological basis of many diseases, with macrophages playing a crucial role in this process. However, research on the role and mechanisms of PBMC in regulating macrophages remains scarce. This study employed an in vitro co-culture model of PBMC and RAW264.7 macrophages to explore the role and mechanisms of PBMC in regulating macrophages. The results showed that the co-culturing led to decreased expression of inflammatory cytokines and increased expression of anti-inflammatory cytokines in RAW264.7 or in the culture supernatant. Additionally, the pro-inflammatory, tissue matrix-degrading M1 macrophages decreased, while the anti-inflammatory, matrix-synthesizing, regenerative M2 macrophages increased in both RAW264.7 and monocytes within PBMC. Moreover, co-cultured macrophages exhibited a significantly decreased p-STAT1/STAT1 ratio, while the p-STAT6/STAT6 ratio significantly increased. This suggests that PBMC may inhibit M1 macrophage polarization by blocking STAT1 signaling cascades and may promote M2 macrophage polarization through the activation of STAT6 signaling cascades. Overall, this study sheds light on the role and mechanism of PBMC in regulating macrophages. Moreover, it was found that monocytes within co-cultured PBMC differentiated into M2 macrophages in the presence of macrophages. This finding provides experimental evidence for the use of PBMC in treating inflammatory diseases, especially macrophage-depleting inflammatory diseases such as osteoarthritis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucocitos Mononucleares / Transducción de Señal / Técnicas de Cocultivo / Factor de Transcripción STAT1 / Factor de Transcripción STAT6 / Macrófagos Límite: Animals Idioma: En Revista: Int Immunopharmacol Asunto de la revista: ALERGIA E IMUNOLOGIA / FARMACOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucocitos Mononucleares / Transducción de Señal / Técnicas de Cocultivo / Factor de Transcripción STAT1 / Factor de Transcripción STAT6 / Macrófagos Límite: Animals Idioma: En Revista: Int Immunopharmacol Asunto de la revista: ALERGIA E IMUNOLOGIA / FARMACOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos