Understanding the role of P-type ATPases in regulating pollen fertility and development in pigeonpea.

Jain, Rishu; Srivastava, Harsha; Kumar, Kuldeep; Sharma, Sandhya; Singh, Anandita; Gaikwad, Kishor

Jain, Rishu; Srivastava, Harsha; Kumar, Kuldeep; Sharma, Sandhya; Singh, Anandita; Gaikwad, Kishor.

Afiliación

Jain R; ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi, 110012, India.
Srivastava H; Department of Biotechnology, TERI School of Advanced Studies, 10 Institutional Area, Vasant Kunj, New Delhi, 110070, India.
Kumar K; ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi, 110012, India.
Sharma S; ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi, 110012, India.
Singh A; ICAR-Indian Institute of Pulses Research, Kanpur, Uttar Pradesh, 208024, India.
Gaikwad K; ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi, 110012, India.

Mol Genet Genomics ; 299(1): 68, 2024 Jul 09.

Article en En | MEDLINE | ID: mdl-38980531

ABSTRACT

ABSTRACT

The P-type ATPase superfamily genes are the cation and phospholipid pumps that transport ions across the membranes by hydrolyzing ATP. They are involved in a diverse range of functions, including fundamental cellular events that occur during the growth of plants, especially in the reproductive organs. The present work has been undertaken to understand and characterize the P-type ATPases in the pigeonpea genome and their potential role in anther development and pollen fertility. A total of 59 P-type ATPases were predicted in the pigeonpea genome. The phylogenetic analysis classified the ATPases into five subfamilies eleven P1B, eighteen P2A/B, fourteen P3A, fifteen P4, and one P5. Twenty-three pairs of P-type ATPases were tandemly duplicated, resulting in their expansion in the pigeonpea genome during evolution. The orthologs of the reported anther development-related genes were searched in the pigeonpea genome, and the expression profiling studies of specific genes via qRT-PCR in the pre- and post-meiotic anther stages of AKCMS11A (male sterile), AKCMS11B (maintainer) and AKPR303 (fertility restorer) lines of pigeonpea was done. Compared to the restorer and maintainer lines, the down-regulation of CcP-typeATPase22 in the post-meiotic anthers of the male sterile line might have played a role in pollen sterility. Furthermore, the strong expression of CcP-typeATPase2 in the post-meiotic anthers of restorer line and CcP-typeATPase46, CcP-typeATPase51, and CcP-typeATPase52 in the maintainer lines, respectively, compared to the male sterile line, clearly indicates their potential role in developing male reproductive organs in pigeonpea.

Asunto(s)

Cajanus; Regulación de la Expresión Génica de las Plantas; Filogenia; Proteínas de Plantas; Polen; Polen/genética; Polen/crecimiento & desarrollo; Cajanus/genética; Cajanus/crecimiento & desarrollo; Cajanus/enzimología; Proteínas de Plantas/genética; Proteínas de Plantas/metabolismo; ATPasas Tipo P/genética; ATPasas Tipo P/metabolismo; Fertilidad/genética; Flores/genética; Flores/crecimiento & desarrollo; Infertilidad Vegetal/genética; Perfilación de la Expresión Génica; Genoma de Planta

Palabras clave

Cytoplasmic male sterility, A2; Hybrid breeding; Maintainer line; Male reproductive organs; P-type ATPases; Restorer line

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Proteínas de Plantas / Polen / Regulación de la Expresión Génica de las Plantas / Cajanus Idioma: En Revista: Mol Genet Genomics Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania

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