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Clinical application of targeted next-generation sequencing in severe pneumonia: a retrospective review.
Zhang, Peng; Liu, Baoyi; Zhang, Shuang; Chang, Xuefei; Zhang, Lihe; Gu, Dejian; Zheng, Xin; Chen, Jiaqing; Xiao, Saiyin; Wu, Zhentao; Cai, Xuemin; Long, Mingfa; Lu, Wenjie; Zheng, Mingzhu; Chen, Rongrong; Gao, Rui; Zheng, Yan; Wu, Jinhua; Feng, Qiujuan; He, Gang; Chen, Yantang; Zheng, Weihao; Zuo, Wanli; Huang, Yanming; Zhang, Xin.
Afiliación
  • Zhang P; Department of Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Liu B; Clinical Experimental Center, Jiangmen Engineering Technology Research Center of Clinical Biobank and Translational Research, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Zhang S; Department of Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Chang X; Department of Respiratory and Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Zhang L; Department of Respiratory and Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Gu D; Geneplus-Beijing Institute, Beijing, 102206, China.
  • Zheng X; Geneplus-Beijing Institute, Beijing, 102206, China.
  • Chen J; Clinical Experimental Center, Jiangmen Engineering Technology Research Center of Clinical Biobank and Translational Research, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Xiao S; Department of Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Wu Z; Department of Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Cai X; Department of Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Long M; Department of Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Lu W; Clinical Experimental Center, Jiangmen Engineering Technology Research Center of Clinical Biobank and Translational Research, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Zheng M; Clinical Experimental Center, Jiangmen Engineering Technology Research Center of Clinical Biobank and Translational Research, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Chen R; Geneplus-Beijing Institute, Beijing, 102206, China.
  • Gao R; Geneplus-Beijing Institute, Beijing, 102206, China.
  • Zheng Y; Department of Research and Development, Guangdong Research Institute of Genetic Diagnostic and Engineering Technologies for Thalassemia, Hybribio Limited, Guangzhou, 510000, China.
  • Wu J; Department of Clinical Laboratory, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Feng Q; Department of Clinical Laboratory, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • He G; Department of Infectious Diseases, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Chen Y; Department of Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Zheng W; Department of Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, 529030, China.
  • Zuo W; Clinical Experimental Center, Jiangmen Engineering Technology Research Center of Clinical Biobank and Translational Research, Jiangmen Central Hospital, Jiangmen, 529030, China. zuowanli@jmszxyy.com.cn.
  • Huang Y; Department of Respiratory and Critical Care Medicine, Jiangmen Central Hospital, Jiangmen, 529030, China. zuowanli@jmszxyy.com.cn.
  • Zhang X; Clinical Experimental Center, Jiangmen Engineering Technology Research Center of Clinical Biobank and Translational Research, Jiangmen Central Hospital, Jiangmen, 529030, China. huangyanming_jxy@163.com.
Crit Care ; 28(1): 225, 2024 Jul 08.
Article en En | MEDLINE | ID: mdl-38978111
ABSTRACT

BACKGROUND:

The precise identification of the underlying causes of infectious diseases, such as severe pneumonia, is essential, and the development of next-generation sequencing (NGS) has enhanced the effectiveness of pathogen detection. However, there is limited information on the systematic assessment of the clinical use of targeted next-generation sequencing (tNGS) in cases of severe pneumonia.

METHODS:

A retrospective analysis was conducted on 130 patients with severe pneumonia treated in the ICU from June 2022 to June 2023. The consistency of the results of tNGS, metagenomics next-generation sequencing (mNGS), and culture with the clinical diagnosis was evaluated. Additionally, the results for pathogens detected by tNGS were compared with those of culture, mNGS, and quantitative reverse transcription PCR (RT-qPCR). To evaluate the efficacy of monitoring severe pneumonia, five patients with complicated infections were selected for tNGS microbiological surveillance. The tNGS and culture drug sensitisation results were then compared.

RESULTS:

The tNGS results for the analysis of the 130 patients showed a concordance rate of over 70% with clinical diagnostic results. The detection of pathogenic microorganisms using tNGS was in agreement with the results of culture, mNGS, and RT-qPCR. Furthermore, the tNGS results for pathogens in the five patients monitored for complicated infections of severe pneumonia were consistent with the culture and imaging test results during treatment. The tNGS drug resistance results were in line with the drug sensitivity results in approximately 65% of the cases.

CONCLUSIONS:

The application of tNGS highlights its promise and significance in assessing the effectiveness of clinical interventions and providing guidance for anti-infection therapies for severe pneumonia.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neumonía / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Crit Care Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neumonía / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Crit Care Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido