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The impact of neutrophil count on the results of metagenomic next-generation sequencing in immunocompromised febrile children.
Wang, Di; Chen, Haipin; Zhao, Cheng; Song, Hua; Zhang, Jingying; Zhao, Fenying; Liang, Juan; Xu, Weiqun; Tang, Yongmin; Xu, Xiaojun.
Afiliación
  • Wang D; Division/Center of Pediatric Hematology-Oncology, Children's Hospital of Zhejiang University School of Medicine, PR China.
  • Chen H; The Pediatric Leukemia Diagnostic and Therapeutic Technology Research Center of Zhejiang Province, National Clinical Research Center for Child Health, PR China.
  • Zhao C; Division/Center of Pediatric Hematology-Oncology, Children's Hospital of Zhejiang University School of Medicine, PR China.
  • Song H; Division/Center of Pediatric Hematology-Oncology, Children's Hospital of Zhejiang University School of Medicine, PR China.
  • Zhang J; Division/Center of Pediatric Hematology-Oncology, Children's Hospital of Zhejiang University School of Medicine, PR China.
  • Zhao F; The Pediatric Leukemia Diagnostic and Therapeutic Technology Research Center of Zhejiang Province, National Clinical Research Center for Child Health, PR China.
  • Liang J; Division/Center of Pediatric Hematology-Oncology, Children's Hospital of Zhejiang University School of Medicine, PR China.
  • Xu W; The Pediatric Leukemia Diagnostic and Therapeutic Technology Research Center of Zhejiang Province, National Clinical Research Center for Child Health, PR China.
  • Tang Y; Division/Center of Pediatric Hematology-Oncology, Children's Hospital of Zhejiang University School of Medicine, PR China.
  • Xu X; The Pediatric Leukemia Diagnostic and Therapeutic Technology Research Center of Zhejiang Province, National Clinical Research Center for Child Health, PR China.
Heliyon ; 10(12): e32816, 2024 Jun 30.
Article en En | MEDLINE | ID: mdl-38975197
ABSTRACT
Metagenomic next-generation sequencing (mNGS) has revolutionized the detection of pathogens, particularly in immunocompromised individuals such as pediatric patients undergoing intensive chemotherapy and hematopoietic stem cell transplantation. This study aims to explore the impact of neutrophil count on the diagnostic efficacy of mNGS in diagnosing infections in pediatric patients with febrile diseases. We conducted a retrospective analysis of pediatric patients with febrile diseases in the hematology/oncology department from January 2019 to September 2022. The study included 387 patients with 516 febrile episodes. Analyzing data from 516 pediatric cases, our study found that 70.7 % had febrile neutropenia (FN) and 29.3 % had febrile without neutropenia (FWN). mNGS demonstrated a high positive detection rate of 84.9 %, compared to 29.7 % for conventional microbiological tests (CMT). While the positive detection rates of mNGS were similar in both FN and FWN groups, bacterial pathogens were more frequently detected in FN patients. Furthermore, the rate of identifying a "probable" microbial etiology was lower in the FN group (46.8 %) compared to the FWN group (65.6 %, p＜0.001). When analyzing the types of organisms and specimens, the "probable" identification rates were particularly lower for viruses and fungi detected by mNGS, as well as in blood and nasopharyngeal swab samples. These findings underscore the significant influence of neutrophil counts on mNGS results in pediatric febrile patients and highlight the necessity for tailored diagnostic approaches in this population.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido