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Studying lipid flip-flop in asymmetric liposomes using 1H NMR and TR-SANS.
Dziura, Dominik; Dziura, Maksymilian; Marquardt, Drew.
Afiliación
  • Dziura D; Department of Chemistry and Biochemistry, University of Windsor, Windsor, ON, Canada.
  • Dziura M; Department of Chemistry and Biochemistry, University of Windsor, Windsor, ON, Canada.
  • Marquardt D; Department of Chemistry and Biochemistry, University of Windsor, Windsor, ON, Canada; Department of Physics, University of Windsor, Windsor, ON, Canada. Electronic address: drew.marquardt@uwindsor.ca.
Methods Enzymol ; 700: 295-328, 2024.
Article en En | MEDLINE | ID: mdl-38971604
ABSTRACT
The specific spatial and temporal distribution of lipids in membranes play a crucial role in determining the biochemical and biophysical properties of the system. In nature, the asymmetric distribution of lipids is a dynamic process with ATP-dependent lipid transporters maintaining asymmetry, and passive transbilayer diffusion, that is, flip-flop, counteracting it. In this chapter, two probe-free techniques, 1H NMR and time-resolved small angle neutron scattering, are described in detail as methods of investigating lipid flip-flop rates in synthetic liposomes that have been generated with an asymmetric bilayer composition.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Difracción de Neutrones / Dispersión del Ángulo Pequeño / Membrana Dobles de Lípidos / Liposomas Idioma: En Revista: Methods Enzymol Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Difracción de Neutrones / Dispersión del Ángulo Pequeño / Membrana Dobles de Lípidos / Liposomas Idioma: En Revista: Methods Enzymol Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos