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Molecular based identification and phylogenetic relationship of the leech Hirudinaria manillensis from India by using mitochondrial cytochrome c oxidase subunit I gene.
Shannan, P Zeebul Trinita; Suganya, Susan G; Ramesh, M; Jemima, E Angel.
Afiliación
  • Shannan PZT; PG and Research Department of Zoology, Bishop Heber College, Affiliated to Bharathidasan University, Tiruchirappalli, Tamilnadu, 620017, India.
  • Suganya SG; PG and Research Department of Zoology, Bishop Heber College, Affiliated to Bharathidasan University, Tiruchirappalli, Tamilnadu, 620017, India. susangsuganya@gmail.com.
  • Ramesh M; Department of Zoology, Thanthai Periyar Government Arts and Science College, Affiliated to Bharathidasan University, Tiruchirappalli, Tamilnadu, 620017, India.
  • Jemima EA; Department of Biotechnology, Trichy Research Institute of Biotechnology, Trichy, India.
Mol Biol Rep ; 51(1): 787, 2024 Jul 06.
Article en En | MEDLINE | ID: mdl-38970720
ABSTRACT

BACKGROUND:

A molecular approach for the identification of unknown species by the using mitochondrial cox1 gene is an effective and reliable as compared with morphological-based identification. Hirudinaria manillensis referred to as Asian Buffalo Leech, is found in South Asia and traditionally used as medicine owing to its medicinal properties. METHODS AND

RESULTS:

The study aimed to isolate and identify the leech species using cox1 gene sequencing and their phylogenetic relationships. The nucleotide sequences of cytochrome c oxidase subunit I (cox1) mitochondrial genes were analyzed for species identification and the phylogenetic relationship of crucial therapeutic leech Hirudinaria manillensis. The isolated DNA from the leech sample was amplified with cox1 gene-specific primers. BLAST results with the H. manillensis sequence showed 89.24% homology with H. manillensis and phylogenetic tree analysis revealed the genetic relationship with other GenBank submitted sequences.

CONCLUSION:

The present study concluded that the cox1 gene could be an effective way to identify the leech H. manillensis and provided sufficient phylogenetic information to distinguish H. manillensis indicating a significant mtDNA-based approach to species identification.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Complejo IV de Transporte de Electrones / Sanguijuelas Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Complejo IV de Transporte de Electrones / Sanguijuelas Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos