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Production of succinate with two CO2 fixation reactions from fatty acids in Cupriavidus necator H16.
Li, Linqing; Zhou, Xiuyuan; Gao, Zhuoao; Xiong, Peng; Liu, Xiutao.
Afiliación
  • Li L; School of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255000, China.
  • Zhou X; International Joint Laboratory on Extremophilic Bacteria and Biological Synthesis, Shandong University of Technology, Zibo, 255000, China.
  • Gao Z; School of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255000, China.
  • Xiong P; International Joint Laboratory on Extremophilic Bacteria and Biological Synthesis, Shandong University of Technology, Zibo, 255000, China.
  • Liu X; International Joint Laboratory on Extremophilic Bacteria and Biological Synthesis, Shandong University of Technology, Zibo, 255000, China.
Microb Cell Fact ; 23(1): 194, 2024 Jul 05.
Article en En | MEDLINE | ID: mdl-38970033
ABSTRACT

BACKGROUND:

Biotransformation of CO2 into high-value-added carbon-based products is a promising process for reducing greenhouse gas emissions. To realize the green transformation of CO2, we use fatty acids as carbon source to drive CO2 fixation to produce succinate through a portion of the 3-hydroxypropionate (3HP) cycle in Cupriavidus necator H16.

RESULTS:

This work can achieve the production of a single succinate molecule from one acetyl-CoA molecule and two CO2 molecules. It was verified using an isotope labeling experiment utilizing NaH13CO3. This implies that 50% of the carbon atoms present in succinate are derived from CO2, resulting in a twofold increase in efficiency compared to prior methods of succinate biosynthesis that relied on the carboxylation of phosphoenolpyruvate or pyruvate. Meanwhile, using fatty acid as a carbon source has a higher theoretical yield than other feedstocks and also avoids carbon loss during acetyl-CoA and succinate production. To further optimize succinate production, different approaches including the optimization of ATP and NADPH supply, optimization of metabolic burden, and optimization of carbon sources were used. The resulting strain was capable of producing succinate to a level of 3.6 g/L, an increase of 159% from the starting strain.

CONCLUSIONS:

This investigation established a new method for the production of succinate by the implementation of two CO2 fixation reactions and demonstrated the feasibility of ATP, NADPH, and metabolic burden regulation strategies in biological carbon fixation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dióxido de Carbono / Ácido Succínico / Cupriavidus necator / Ácidos Grasos Idioma: En Revista: Microb Cell Fact Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dióxido de Carbono / Ácido Succínico / Cupriavidus necator / Ácidos Grasos Idioma: En Revista: Microb Cell Fact Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido