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Hyaluronic acid impacts hematological endpoints and spleen histological features in African catfish (Clarias gariepinus).
Hamed, Mohamed; Kotob, Mohamed H; Abou Khalil, Nasser S; Anwari, Esraa A; El Gazzar, Walaa Bayoumie; Idriss, Shaimaa K A; Fakhry, Michel E; Farag, Amina A; Sabra, Mahmoud S; Salaah, Sally M; Abdel-Zaher, Souzan; Yehia Saad, Fatma Alzahraa; Naguib, Mervat; Lee, Jae-Seong; Sayed, Alaa El-Din H.
Afiliación
  • Hamed M; Department of Zoology, Faculty of Science, Al-Azhar University (Assiut Branch), Assiut, 71524, Egypt.
  • Kotob MH; Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Skip Bertman Drive, Baton Rouge, LA, 70803, USA.
  • Abou Khalil NS; Department of Pathology, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt.
  • Anwari EA; Division of Pharmacology and Toxicology, Department of Pharmaceutical Sciences, University of Vienna, Vienna, 1090, Austria.
  • El Gazzar WB; Department of Animal Physiology and Biochemistry, Faculty of Veterinary Medicine, Badr University, Assuit, Egypt.
  • Idriss SKA; Department of Medical Physiology, Faculty of Medicine, Assuit University, Assiut, 71516, Egypt.
  • Fakhry ME; Department of Zoology, Faculty of Science, Assiut University, Assiut, 71516, Egypt.
  • Farag AA; Department of Anatomy, Physiology and Biochemistry, Faculty of Medicine, the Hashemite University, Zarqa, 13133, Jordan.
  • Sabra MS; 9Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Benha University, Benha City, 13518, Egypt.
  • Salaah SM; Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Assiut University, Assiut, 71516, Egypt.
  • Abdel-Zaher S; Department of Medical Biochemistry and molecular biology, Faculty of Medicine, Assiut University, Assiut, 71516, Egypt.
  • Yehia Saad FA; Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Benha University, Benha City, 13518, Egypt.
  • Naguib M; Department of Pharmacology, Faculty of Veterinary Medicine, Assiut University, Assiut, 71516, Egypt.
  • Lee JS; Fresh Water Division, National Institute of Oceanography and Fisheries, NIOF, Cairo, Egypt.
  • Sayed AEH; Department of Molecular Biology, Molecular Biology Research & Studies Institute, Assiut University, Assiut, 71516, Egypt.
BMC Vet Res ; 20(1): 294, 2024 Jul 05.
Article en En | MEDLINE | ID: mdl-38970005
ABSTRACT
Since its identification in the vitreous humour of the eye and laboratory biosynthesis, hyaluronic acid (HA) has been a vital component in several pharmaceutical, nutritional, medicinal, and cosmetic uses. However, little is known about its potential toxicological impacts on aquatic inhabitants. Herein, we investigated the hematological response of Clarias gariepinus to nominal doses of HA. To achieve this objective, 72 adult fish were randomly and evenly distributed into four groups control, low-dose (0.5 mg/l HA), medium-dose (10 mg/l HA), and high-dose (100 mg/l HA) groups for two weeks each during both the exposure and recovery periods. The findings confirmed presence of anemia, neutrophilia, leucopoenia, lymphopenia, and eosinophilia at the end of exposure to HA. In addition, poikilocytosis and a variety of cytomorphological disturbances were observed. Dose-dependent histological alterations in spleen morphology were observed in the exposed groups. After HA removal from the aquarium for 2 weeks, the groups exposed to the two highest doses still exhibited a notable decline in red blood cell count, hemoglobin concentration, mean corpuscular hemoglobin concentration, and an increase in mean corpuscular volume. Additionally, there was a significant rise in neutrophils, eosinophils, cell alterations, and nuclear abnormalities percentages, along with a decrease in monocytes, coupled with a dose-dependent decrease in lymphocytes. Furthermore, only the highest dose of HA in the recovered groups continued to cause a significant increase in white blood cells. White blood cells remained lower, and the proportion of apoptotic RBCs remained higher in the high-dose group. The persistence of most of the haematological and histological disorders even after recovery period indicates a failure of physiological compensatory mechanisms to overcome the HA-associated problems or insufficient duration of recovery. Thus, these findings encourage the inclusion of this new hazardous agent in the biomonitoring program and provide a specific pattern of hematological profile in HA-challenged fish. Further experiments are highly warranted to explore other toxicological hazards of HA using dose/time window protocols.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bazo / Bagres / Ácido Hialurónico Límite: Animals Idioma: En Revista: BMC Vet Res Asunto de la revista: MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bazo / Bagres / Ácido Hialurónico Límite: Animals Idioma: En Revista: BMC Vet Res Asunto de la revista: MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Reino Unido