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Dual-reporter fluorescent probe for precise identification of liver cancer by sequentially responding to carboxylesterase and polarity.
Rong, Xiaodi; Li, Xiwei; Liu, Caiyun; Wu, Chuanchen; Wang, Zhongpeng; Zhu, Baocun.
Afiliación
  • Rong X; School of Water Conservancy and Environment, University of Jinan, Jinan, 250022, China.
  • Li X; College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institutes of Biomedical Sciences, Shandong Normal Universi
  • Liu C; School of Water Conservancy and Environment, University of Jinan, Jinan, 250022, China. Electronic address: liucaiyun1982072@163.com.
  • Wu C; College of Medicine, Linyi University, Linyi, 276005, China. Electronic address: wuchuanchen@lyu.edu.cn.
  • Wang Z; School of Water Conservancy and Environment, University of Jinan, Jinan, 250022, China.
  • Zhu B; School of Water Conservancy and Environment, University of Jinan, Jinan, 250022, China. Electronic address: lcyzbc@163.com.
Talanta ; 278: 126477, 2024 Oct 01.
Article en En | MEDLINE | ID: mdl-38968656
ABSTRACT
Early treatment significantly improves the survival rate of liver cancer patients, so the development of early diagnostic methods for liver cancer is urgent. Liver cancer can develop from viral hepatitis, alcoholic liver, and fatty liver, thus making the above diseases share common features such as elevated viscosity, reactive oxygen species, and reactive nitrogen species. Therefore, accurate differentiation between other liver diseases and liver cancer is both a paramount practical need and challenging. Numerous fluorescent probes have been reported for the diagnosis of liver cancer by detecting a single biomarker, but these probes lack specificity for liver cancer in complex biological systems. Obviously, using multiple liver cancer biomarkers as the basis for judgment can dramatically improve diagnostic accuracy. Herein, we report the first fluorescent probe, LD-TCE, that sequentially detects carboxylesterase (CE) and lipid droplet polarity in liver cancer cells with high sensitivity and selectivity, with linear detection of CE in the range of 0-6 U/mL and a 65-fold fluorescence enhancement in response to polarity. The probe first reacts with CE and releases weak fluorescence, which is then dramatically enhanced due to the decrease in lipid droplet polarity in liver cancer cells. This approach allows the probe to enable specific imaging of liver cancer with higher contrast and accuracy. The probe successfully achieved the screening of liver cancer cells and the precise identification of liver cancer in mice. More importantly, it is not disturbed by liver fibrosis, which is a common pathological feature of many liver diseases. We believe that the LD-TCE is expected to be a powerful tool for early diagnosis of liver cancer.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carboxilesterasa / Colorantes Fluorescentes / Neoplasias Hepáticas Límite: Animals / Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carboxilesterasa / Colorantes Fluorescentes / Neoplasias Hepáticas Límite: Animals / Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos