H19 lncRNA triggers ferroptosis, exacerbating ox-LDL-induced artery endothelial cell damage in vitro.
Clin Hemorheol Microcirc
; 88(2): 263-275, 2024.
Article
en En
| MEDLINE
| ID: mdl-38968045
ABSTRACT
BACKGROUND:
The precise association between lncRNA H19 and ferroptosis in the context of atherosclerosis remains uncertain.OBJECTIVE:
This study is to clarify the underlying process and propose novel approaches for the advancement of therapeutic interventions targeting atherosclerosis.METHODS:
Assessment of ferroptosis, which entails the evaluation of cell viability using CCK-8 and the quantification of intracellular MDA, GSH, and ferrous ions. Simultaneously, the protein expression levels of assessed by western blot analysis, while the expression level of lncRNA H19 was also determined. Furthermore, HAECs that were cultured with ox-LDL were subjected to Fer-1 interference. HAECs were exposed to ox-LDL and then transfected with H19 shRNA and H19 overexpression vector pcDNA3.1. The level of ferroptosis in the cells was then measured. Then, HAECs were subjected to incubation with ox-LDL, followed by transfection with H19 shRNA and treated with Erastin to assess the levels of ferroptosis, cell viability, and inflammatory factor production. and the ability for blood vessel development.RESULTS:
The survival rate of HAECs in the ox-LDL group was much lower. Ox-LDL resulted in an upregulation of ACSL4 expression in HAECs, while the expression of SLC7A11 and GPX4 decreased.CONCLUSIONS:
lncRNA H19 enhances ferroptosis and exacerbates arterial endothelial cell damage induced by LDL.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Endoteliales
/
ARN Largo no Codificante
/
Ferroptosis
/
Lipoproteínas LDL
Límite:
Humans
Idioma:
En
Revista:
Clin Hemorheol Microcirc
Asunto de la revista:
ANGIOLOGIA
/
HEMATOLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Países Bajos