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LncRNA SNHG12 suppresses adipocyte inflammation and insulin resistance by regulating the HDAC9/Nrf2 axis.
Huang, Xiaoyan; Chen, Jixiong; Li, Haidan; Cai, Yuhua; Liu, Li; Dong, Qi; Li, Yan; Ren, Yi; Xiang, Wei; He, Xiaojie.
Afiliación
  • Huang X; Department of Genetics, Metabolism and Endocrinology, Hainan Women and Children's Medical Center, Haikou, China.
  • Chen J; Department of Medical Care Center, Hainan Provincial People's Hospital, Haikou, China.
  • Li H; Department of Genetics, Metabolism and Endocrinology, Hainan Women and Children's Medical Center, Haikou, China.
  • Cai Y; Department of Genetics, Metabolism and Endocrinology, Hainan Women and Children's Medical Center, Haikou, China.
  • Liu L; Department of Genetics, Metabolism and Endocrinology, Hainan Women and Children's Medical Center, Haikou, China.
  • Dong Q; Department of Genetics, Metabolism and Endocrinology, Hainan Women and Children's Medical Center, Haikou, China.
  • Li Y; Department of Genetics, Metabolism and Endocrinology, Hainan Women and Children's Medical Center, Haikou, China.
  • Ren Y; Department of Pediatrics, Haikou Hospital of the Maternal and Child Health, Haikou, China.
  • Xiang W; Hainan Women and Children's Medical Center, Haikou, China.
  • He X; Laboratory of Pediatric Nephrology, Institute of Pediatrics, The Second Xiangya Hospital, Central South University, Changsha, China.
FASEB J ; 38(13): e23794, 2024 Jul 15.
Article en En | MEDLINE | ID: mdl-38967258
ABSTRACT
Obesity is often associated with low-grade inflammation. The incidence of obesity has increased annually worldwide, which seriously affects human health. A previous study indicated that long noncoding RNA SNHG12 was downregulated in obesity. Nevertheless, the role of SNHG12 in obesity remains to be elucidated. In this study, qRT-PCR, western blot, and ELISA were utilized to examine the gene and protein expression. Flow cytometry was employed to investigate the M2 macrophage markers. RNA pull-down assay and RIP were utilized to confirm the interactions of SNHG12, hnRNPA1, and HDAC9. Eventually, a high-fat diet-fed mouse model was established for in vivo studies. SNHG12 overexpression suppressed adipocyte inflammation and insulin resistance and promoted M2 polarization of macrophages that was caused by TNF-α treatment. SNHG12 interacted with hnRNPA1 to downregulate HDAC9 expression, which activated the Nrf2 signaling pathway. HDAC9 overexpression reversed the effect of SNHG12 overexpression on inflammatory response, insulin resistance, and M2 phenotype polarization. Overexpression of SNHG12 improved high-fat diet-fed mouse tissue inflammation. This study revealed the protective effect of SNHG12 against adipocyte inflammation and insulin resistance. This result further provides a new therapeutic target for preventing inflammation and insulin resistance in obesity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Represoras / Resistencia a la Insulina / Adipocitos / Factor 2 Relacionado con NF-E2 / Dieta Alta en Grasa / ARN Largo no Codificante / Histona Desacetilasas / Inflamación / Ratones Endogámicos C57BL / Obesidad Límite: Animals Idioma: En Revista: FASEB J Asunto de la revista: BIOLOGIA / FISIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Represoras / Resistencia a la Insulina / Adipocitos / Factor 2 Relacionado con NF-E2 / Dieta Alta en Grasa / ARN Largo no Codificante / Histona Desacetilasas / Inflamación / Ratones Endogámicos C57BL / Obesidad Límite: Animals Idioma: En Revista: FASEB J Asunto de la revista: BIOLOGIA / FISIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos