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Production of a new chitinase from Nocardiopsis halophila TN-X8 utilizing bio-waste from the blue swimming crab: enzyme characterization and immobilization.
Mechri, Sondes; Jabeur, Fadoua; Bessadok, Boutheina; Moumnassi, Sara; Idrissi Yahyaoui, Meryem; Mannani, Nysrine; Asehraou, Abdeslam; Mensi, Fethi; Vita, Stefano; D'Amore, Paolo; Di Bella, Calogero; Lo Monaco, Daniela; Abousalham, Abdelkarim; Sadok, Saloua; Le Roes-Hill, Marilize; Jaouadi, Bassem.
Afiliación
  • Mechri S; Laboratory of Microbial and Enzymatic Biotechnologies and Biomolecules (LMEBB), Centre of Biotechnology of Sfax (CBS), University of Sfax (USF), Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.
  • Jabeur F; Laboratory of Microbial and Enzymatic Biotechnologies and Biomolecules (LMEBB), Centre of Biotechnology of Sfax (CBS), University of Sfax (USF), Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia.
  • Bessadok B; Laboratory of Blue Biotechnology and Aquatic Bioproducts (B3Aqua), Institut National des Sciences et Technologies de la Mer (INSTM), Annexe La Goulette Port de Pêche, 2060, La Goulette, Tunisia.
  • Moumnassi S; Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health (LBBEH), Faculty of Sciences of Oujda (FSO), Mohammed Premier University (UMP), BV Mohamed VI, P.O. Box 717, 60000, Oujda, Morocco.
  • Idrissi Yahyaoui M; Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health (LBBEH), Faculty of Sciences of Oujda (FSO), Mohammed Premier University (UMP), BV Mohamed VI, P.O. Box 717, 60000, Oujda, Morocco.
  • Mannani N; Laboratory of Marine Biotechnologies and Environment (BIOMARE), Faculty of Sciences of El Jadida (FS El Jadida), Chouaib Doukkali University (UCD), Road of Ben Maachou, P.O. Box 20, 24000, El Jadida, Morocco.
  • Asehraou A; Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health (LBBEH), Faculty of Sciences of Oujda (FSO), Mohammed Premier University (UMP), BV Mohamed VI, P.O. Box 717, 60000, Oujda, Morocco.
  • Mensi F; Laboratory of Blue Biotechnology and Aquatic Bioproducts (B3Aqua), Institut National des Sciences et Technologies de la Mer (INSTM), Annexe La Goulette Port de Pêche, 2060, La Goulette, Tunisia.
  • Vita S; Instituto Zooprofilattico Sperimentale Della Sicilia (IZSSi), Via G. Marinuzzi, 3, 90129, Palermo, Italy.
  • D'Amore P; Instituto Zooprofilattico Sperimentale Della Sicilia (IZSSi), Via G. Marinuzzi, 3, 90129, Palermo, Italy.
  • Di Bella C; Instituto Zooprofilattico Sperimentale Della Sicilia (IZSSi), Via G. Marinuzzi, 3, 90129, Palermo, Italy.
  • Lo Monaco D; Instituto Zooprofilattico Sperimentale Della Sicilia (IZSSi), Via G. Marinuzzi, 3, 90129, Palermo, Italy.
  • Abousalham A; Institut de Chimie et de Biochimie Moléculaires et Supramoléculaires (ICBMS), UMR 5246 CNRS, Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires (GEMBAS), Univ Lyon, Université Lyon 1, Bât Raulin, 43 Bd du 11 Novembre 1918, 69622, Villeurbanne Cedex, France.
  • Sadok S; Laboratory of Blue Biotechnology and Aquatic Bioproducts (B3Aqua), Institut National des Sciences et Technologies de la Mer (INSTM), Annexe La Goulette Port de Pêche, 2060, La Goulette, Tunisia.
  • Le Roes-Hill M; Applied Microbial and Health Biotechnology Institute (AMHBI), Cape Peninsula University of Technology, P.O. Box 1906, Bellville, 7535, South Africa.
  • Jaouadi B; Laboratory of Microbial and Enzymatic Biotechnologies and Biomolecules (LMEBB), Centre of Biotechnology of Sfax (CBS), University of Sfax (USF), Road of Sidi Mansour Km 6, P.O. Box 1177, 3018, Sfax, Tunisia. bassem.jaouadi@cbs.rnrt.tn.
Environ Sci Pollut Res Int ; 31(32): 45217-45233, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38958861
ABSTRACT
In accordance with the framework of the Circular Blue Bioeconomy in the Mediterranean region, the objective of this study was to evaluate the biotransformation of blue swimming crab (Portunus segnis) residues obtained from the port of Sfax by an extracellular chitinase produced by Nocardiopsis halophila strain TN-X8 isolated from Chott El Jerid (Tozeur, Tunisia). From the analysis of multiple extremophilic Actinomycetota, it was determined that strain TN-X8 exclusively utilized 60 g/L of raw blue swimming crab as its carbon and energy source, achieving a chitinase activity of approximately 950 U/mL following a 6-day incubation period at 40 °C. Pure chitinase, designated as ChiA-Nh30, was obtained after heat treatment, followed by ammonium sulfate fractionation and Sephacryl® S-200 column chromatography. The maximum ChiA-Nh30 activity was observed at pH 3 and 75 °C. Interestingly, compared with cyclohexamidine, ChiA-Nh30 showed a good antifungal effect against four pathogenic fungi. Furthermore, when using colloidal chitin as substrate, ChiA-Nh30 demonstrated a higher degree of catalytic efficiency than the commercially available Chitodextrinase®. In addition, ChiA-Nh30 could be immobilized by applying encapsulation and encapsulation-adsorption techniques. The kaolin and charcoal used acted as excellent binders, resulting in improved ChiA-Nh30 stability. For the immobilized ChiA-Nh30, the yield of N-acetyl-D-glucosamine monomers released from 20% (w/v) blue swimming crab residues increased by 3.1 (kaolin) and 2.65 (charcoal) times, respectively.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quitinasas / Braquiuros Límite: Animals Idioma: En Revista: Environ Sci Pollut Res Int Asunto de la revista: SAUDE AMBIENTAL / TOXICOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quitinasas / Braquiuros Límite: Animals Idioma: En Revista: Environ Sci Pollut Res Int Asunto de la revista: SAUDE AMBIENTAL / TOXICOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Túnez Pais de publicación: Alemania